Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres
| العنوان: | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres |
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| المؤلفون: | Luân C. Nguyễn, Kenta Masuda, Ahmed G. K. Habib, Tomoko Nanbu, Naoya Hirata, Masashi Yukawa, Daiki Tanaka, Eiko Tsuchiya, Shinobu Ukimori, Masaru Ueno, Katsunori Takahashi |
| المصدر: | PLoS ONE, Vol 10, Iss 10, p e0140456 (2015) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Flap Endonucleases, Telomere-Binding Proteins, lcsh:Medicine, Shelterin Complex, Schizosaccharomyces, Flap endonuclease, lcsh:Science, Uncapping, Telomere-binding protein, Multidisciplinary, Microbial Viability, biology, lcsh:R, Fungal genetics, DNA Helicases, Helicase, Telomere, biology.organism_classification, Molecular biology, Cell biology, Exodeoxyribonucleases, Schizosaccharomyces pombe, biology.protein, lcsh:Q, Schizosaccharomyces pombe Proteins, Chromosomes, Fungal, Gene Deletion, Research Article |
| الوصف: | The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair. This research is (partially) supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Platform for Dynamic Approaches to Living System) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and Japan Agency for Medical Research and Development (AMED), and by Grants-in-Aid for Scientific Research in Priority Areas, provided to M. U. from the Ministry of Education, Culture, Sports, Science and Technology, Japan. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce57b0cb3b863d771a24cddef3ab58c4Test http://europepmc.org/articles/PMC4605587?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ce57b0cb3b863d771a24cddef3ab58c4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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