Phosphorylation of AATYK1 by Cdk5 Suppresses Its Tyrosine Phosphorylation
| العنوان: | Phosphorylation of AATYK1 by Cdk5 Suppresses Its Tyrosine Phosphorylation |
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| المؤلفون: | Tetsuya Takano, Mitsunori Fukuda, Ryo Endo, Koji Tsutsumi, Mineko Tomomura, Toshio Ohshima, Shin-ichi Hisanaga |
| المصدر: | PLoS ONE, Vol 5, Iss 4, p e10260 (2010) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cell Biology/Neuronal Signaling Mechanisms, lcsh:Medicine, Endosomes, Protein tyrosine phosphatase, Receptor tyrosine kinase, MAP2K7, Mice, chemistry.chemical_compound, Cell Biology/Membranes and Sorting, Chlorocebus aethiops, Biochemistry/Cell Signaling and Trafficking Structures, Neuroscience/Neuronal Signaling Mechanisms, Animals, Protein phosphorylation, Phosphorylation, lcsh:Science, Cells, Cultured, MAPK14, Neurons, Multidisciplinary, biology, lcsh:R, Brain, Cyclin-Dependent Kinase 5, Tyrosine phosphorylation, Protein-Tyrosine Kinases, Molecular biology, Cell biology, src-Family Kinases, nervous system, chemistry, COS Cells, biology.protein, Tyrosine, lcsh:Q, Apoptosis Regulatory Proteins, Research Article, Protein Binding, Proto-oncogene tyrosine-protein kinase Src |
| الوصف: | Apoptosis-associated tyrosine kinase 1 (AATYK1), a novel serine/threonine kinase that is highly expressed in the brain, is involved in neurite extension and apoptosis of cerebellar granule neurons; however, its precise function remains unknown. In this study, we investigated the interaction of AATYK1A with Cyclin-dependent kinase 5 (Cdk5)/p35, a proline-directed protein kinase that is predominantly expressed in neurons. AATYK1A bound to the p35 activation subunit of Cdk5 in cultured cells and in mouse brains and colocalized with p35 on endosomes in COS-7 cells. AATYK1A was phosphorylated at Ser34 by Cdk5/p35 in vitro, in cultured neurons and in mouse brain. In PC12D cells, Ser34 phosphorylation increased after treatment with nerve growth factor and phosphorylated AATYK1A accumulated in growth cones of PC12D cells. Ser34 phosphorylation suppressed the tyrosine phosphorylation of AATYK1A by Src family kinases. These results suggest a possibility that AATYK1A plays a role in early to recycling endosomes and its function is regulated by phosphorylation with Cdk5 or Src-family kinases. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e806fb72046fe194ba116a755c5af07Test https://doi.org/10.1371/journal.pone.0010260Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5e806fb72046fe194ba116a755c5af07 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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