Electron Transfer Flavoprotein Subunit Beta Is a Candidate Endothelial Cell Autoantigen in Behçet's Disease
| العنوان: | Electron Transfer Flavoprotein Subunit Beta Is a Candidate Endothelial Cell Autoantigen in Behçet's Disease |
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| المؤلفون: | Guangyu Chen, Yabin Zhou, Weikang Yang, Fuxin Ma, Chunhe Yang, Peng Chen, Yiping Xun, Shutao Sun, ChunYan Zhang, Yaping Tian, Hongwu Du, Lili Shi, Lanqing Zhao |
| المصدر: | PLoS ONE, Vol 10, Iss 4, p e0124760 (2015) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Adolescent, Electron-Transferring Flavoproteins, Protein subunit, Gene Expression, lcsh:Medicine, Behcet's disease, Autoantigens, Electron-transferring flavoprotein, law.invention, Young Adult, Antigen, law, Human Umbilical Vein Endothelial Cells, medicine, Humans, Amino Acid Sequence, lcsh:Science, Aged, Multidisciplinary, biology, Behcet Syndrome, lcsh:R, Autoantibody, Endothelial Cells, Middle Aged, medicine.disease, Immunohistochemistry, Molecular biology, Peptide Fragments, Blot, biology.protein, Recombinant DNA, Female, lcsh:Q, Antibody, Research Article |
| الوصف: | Behçet's disease (BD) is a chronic inflammatory disease with multisystem involvement, and it is listed as a rare disease in the United States but is common in the Middle East, China, and Japan. The aim of this study was to identify novel autoantigens in Chinese patients with BD. First, the candidate autoantigens were screened by Western blotting, and the sequences of putative antigens were identified by LC-MALDI-TOF/TOF mass spectrometry. Next, the screened protein was cloned, expressed and purified. Then, an optimized ELISA was developed, and the serological criteria were evaluated using a large number of confirmed patients. One antigen with a molecular weight of approximately 28 kDa was identified as electron transfer flavoprotein subunit beta (ETFB). Positive reactivity was detected in recombinant human ETFB sera from 38 of 92 BD patients (41 %) and 1 of 90 healthy controls (1 %). |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7237d32460c12188268ce070023cc84Test http://europepmc.org/articles/PMC4410958?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a7237d32460c12188268ce070023cc84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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