The Neuronal PAS Domain Protein 4 (Npas4) Is Required for New and Reactivated Fear Memories
| العنوان: | The Neuronal PAS Domain Protein 4 (Npas4) Is Required for New and Reactivated Fear Memories |
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| المؤلفون: | Ralph J. DiLeone, Glenn E. Schafe, Jonathan E. Ploski, Tam Nguyen, Melissa S. Monsey |
| المصدر: | PLoS ONE, Vol 6, Iss 8, p e23760 (2011) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Conditioning, Classical, Gene Expression, lcsh:Medicine, Biology, Social and Behavioral Sciences, Signaling Pathways, Amygdala, Molecular Genetics, Rats, Sprague-Dawley, Behavioral Neuroscience, 03 medical and health sciences, Learning and Memory, 0302 clinical medicine, Memory, PAS domain, Molecular Cell Biology, Genetics, Basic Helix-Loop-Helix Transcription Factors, medicine, Psychology, Animals, Learning, Fear conditioning, lcsh:Science, 030304 developmental biology, Fear processing in the brain, 0303 health sciences, Multidisciplinary, Behavior, Animal, Long-term memory, lcsh:R, Cognitive Psychology, Computational Biology, Fear, Rats, Mental Health, medicine.anatomical_structure, Synaptic plasticity, Medicine, lcsh:Q, Memory consolidation, Molecular Neuroscience, Immediate early gene, Neuroscience, 030217 neurology & neurosurgery, Research Article |
| الوصف: | The Neuronal PAS domain protein 4 (Npas4) is a neuronal activity-dependent immediate early gene that has recently been identified as a transcription factor which regulates the transcription of genes that control inhibitory synapse development and synaptic plasticity. The role Npas4 in learning and memory, however, is currently unknown. Here, we systematically examine the role of Npas4 in auditory Pavlovian fear conditioning, an amygdala-dependent form of emotional learning. In our first series of experiments, we show that Npas4 mRNA and protein are regulated in the rat lateral nucleus of the amygdala (LA) in a learning-dependent manner. Further, knockdown of Npas4 protein in the LA via adeno-associated viral (AAV) mediated gene delivery of RNAi was observed to impair fear memory formation, while innate fear and the expression of fear memory were not affected. In our second series of experiments, we show that Npas4 protein is regulated in the LA by retrieval of an auditory fear memory and that knockdown of Npas4 in the LA impairs retention of a reactivated, but not a non-reactivated, fear memory. Collectively, our findings provide the first comprehensive look at the functional role of Npas4 in learning and memory. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a6394efe4ef6be58a8249ae1eed1e2cTest https://doi.org/10.1371/journal.pone.0023760Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3a6394efe4ef6be58a8249ae1eed1e2c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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