دورية أكاديمية
Cooperative Nuclear Localization Sequences Lend a Novel Role to the N-Terminal Region of MSH6
| العنوان: | Cooperative Nuclear Localization Sequences Lend a Novel Role to the N-Terminal Region of MSH6 |
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| المؤلفون: | Gassman, Natalie R., Clodfelter, Jill E., McCauley, Anita K., Bonin, Keith, Salsbury, Freddie R., Jr., Scarpinato, Karin D. |
| المصدر: | PLoS ONE |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2011 |
| المجموعة: | Wake Forest University: WakeSpace Digital Archive |
| مصطلحات موضوعية: | biophysics, molecular biology |
| الوصف: | Human mismatch repair proteins MSH2-MSH6 play an essential role in maintaining genetic stability and preventing disease. While protein functions have been extensively studied, the substantial amino-terminal region (NTR*) of MSH6 that is unique to eukaryotic proteins, has mostly evaded functional characterization. We demonstrate that a cluster of three nuclear localization signals (NLS) in the NTR direct nuclear import. Individual NLSs are capable of partially directing cytoplasmic protein into the nucleus; however only cooperative effects between all three NLSs efficiently transport MSH6 into the nucleus. In striking contrast to yeast and previous assumptions on required heterodimerization, human MSH6 does not determine localization of its heterodimeric partner, MSH2. A cancer-derived mutation localized between two of the three NLS significantly decreases nuclear localization of MSH6, suggesting altered protein localization can contribute to carcinogenesis. These results clarify the pending speculations on the functional role of the NTR in human MSH6 and identify a novel, cooperative nuclear localization signal. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Gassman NR, Clodfelter JE, McCauley AK, Bonin K, Salsbury FR Jr, et al. (2011) Cooperative Nuclear Localization Sequences Lend a Novel Role to the N-Terminal Region of MSH6. PLoS ONE 6(3): e17907. doi:10.1371/journal.pone.0017907; http://hdl.handle.net/10339/37399Test; http://dx.doi.org/10.1371/journal.pone.0017907Test |
| DOI: | 10.1371/journal.pone.0017907 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0017907Test http://hdl.handle.net/10339/37399Test |
| رقم الانضمام: | edsbas.ED1F19AD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0017907 |
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