المؤلفون: Melody Cools, Simon Lissoir, Elisabeth Bodo, Judith Ulloa-Calzonzin, Alexander DeLuna, Isabelle Georis, Bruno André

المصدر: PLoS Genetics, Vol 16, Iss 8, p e1008966 (2020)

مصطلحات موضوعية: Genetics, QH426-470

الوصف: The vacuole of the yeast Saccharomyces cerevisiae plays an important role in nutrient storage. Arginine, in particular, accumulates in the vacuole of nitrogen-replete cells and is mobilized to the cytosol under nitrogen starvation. The arginine import and export systems involved remain poorly characterized, however. Furthermore, how their activity is coordinated by nitrogen remains unknown. Here we characterize Vsb1 as a novel vacuolar membrane protein of the APC (amino acid-polyamine-organocation) transporter superfamily which, in nitrogen-replete cells, is essential to active uptake and storage of arginine into the vacuole. A shift to nitrogen starvation causes apparent inhibition of Vsb1-dependent activity and mobilization of stored vacuolar arginine to the cytosol. We further show that this arginine export involves Ypq2, a vacuolar protein homologous to the human lysosomal cationic amino acid exporter PQLC2 and whose activity is detected only in nitrogen-starved cells. Our study unravels the main arginine import and export systems of the yeast vacuole and suggests that they are inversely regulated by nitrogen.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1553-7390Test; https://doaj.org/toc/1553-7404Test

الوصول الحر: https://doaj.org/article/27379f458c424684ad1259280e64a93dTest

المؤلفون: Aria Ronsmans, Maxime Wery, Ugo Szachnowski, Camille Gautier, Marc Descrimes, Evelyne Dubois, Antonin Morillon, Isabelle Georis

المصدر: PLoS Genetics, Vol 15, Iss 2, p e1007999 (2019)

مصطلحات موضوعية: Genetics, QH426-470

الوصف: GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites, correlating with nitrogen- and/or Dal80-sensitive gene expression. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies requires active transcription. Consistently, Dal80 co-immunoprecipitated with the initiating and post-initiation forms of RNA Polymerase II. Our work suggests that GATA factors could play dual, synergistic roles during transcription initiation and post-initiation steps, promoting efficient remodeling of the gene expression program in response to environmental changes.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC6413948?pdf=renderTest; https://doaj.org/toc/1553-7390Test; https://doaj.org/toc/1553-7404Test

الوصول الحر: https://doaj.org/article/06c1ca71de1a409e8f17b0b2daad6779Test

المؤلفون: Marc Descrimes, Ugo Szachnowski, Maxime Wery, Camille Gautier, Antonin Morillon, Evelyne Dubois, Aria Ronsmans, Isabelle Georis

المساهمون: Université Libre de Bruxelles [Bruxelles] (ULB), Dynamique de l'information génétique : bases fondamentales et cancer (DIG CANCER), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie-Centre National de la Recherche Scientifique (CNRS), Institut Curie-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Œstrogènes, reproduction, cancer (OeReCa), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Université libre de Bruxelles (ULB), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Sorbonne Université (SU)

المصدر: PLOS genetics, 15 (2
PLoS Genetics
PLoS Genetics, Public Library of Science, 2019, 15 (2), pp.e1007999. ⟨10.1371/journal.pgen.1007999⟩
PLoS Genetics, Vol 15, Iss 2, p e1007999 (2019)

مصطلحات موضوعية: Cancer Research, Génétique clinique, Transcription, Genetic, Chi square tests, RNA polymerase II, Gene prediction, Biochemistry, GATA Transcription Factors, Database and Informatics Methods, Mathematical and Statistical Techniques, 0302 clinical medicine, Transcription (biology), Gene Expression Regulation, Fungal, Gene expression, DNA, Fungal, Promoter Regions, Genetic, ComputingMilieux_MISCELLANEOUS, Genetics (clinical), Regulation of gene expression, 0303 health sciences, Statistics, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Genomics, Up-Regulation, Cell biology, Physical Sciences, RNA Polymerase II, Sequence Analysis, Biologie, Research Article, Chromatin Immunoprecipitation, Saccharomyces cerevisiae Proteins, lcsh:QH426-470, Bioinformatics, Genetic loci, DNA transcription, Saccharomyces cerevisiae, Evolution des espèces, Biology, Research and Analysis Methods, 03 medical and health sciences, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], DNA-binding proteins, Transcription factors, Genetics, Statistical Methods, Statistical Hypothesis Testing, Molecular Biology, Transcription factor, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Binding Sites, Biology and life sciences, Ecologie, Sequence Analysis, RNA, Computational Biology, Proteins, Biologie moléculaire, Promoter, Genome Analysis, Regulatory Proteins, Gene regulation, Repressor Proteins, Cancérologie, lcsh:Genetics, Sequence motif analysis, biology.protein, GATA transcription factor, Mathematics, 030217 neurology & neurosurgery

الوصف: GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites, correlating with nitrogen- and/or Dal80-sensitive gene expression. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies requires active transcription. Consistently, Dal80 co-immunoprecipitated with the initiating and post-initiation forms of RNA Polymerase II. Our work suggests that GATA factors could play dual, synergistic roles during transcription initiation and post-initiation steps, promoting efficient remodeling of the gene expression program in response to environmental changes.
SCOPUS: ar.j
info:eu-repo/semantics/published

وصف الملف: 1 full-text file(s): application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2a94513318d1c605355b8d16bd0ac87Test
https://doi.org/10.1371/journal.pgen.1007999Test