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1دورية أكاديمية
المؤلفون: Xu Liu, Zhiyuan Yao, Meiyan Jin, Sim Namkoong, Zhangyuan Yin, Jun Hee Lee, Daniel J Klionsky
المصدر: PLoS Biology, Vol 17, Iss 4, p e3000219 (2019)
مصطلحات موضوعية: Biology (General), QH301-705.5
الوصف: Macroautophagy (hereafter autophagy) is a well-conserved cellular process through which cytoplasmic components are delivered to the vacuole/lysosome for degradation and recycling. Studies have revealed the molecular mechanism of transcriptional regulation of autophagy-related (ATG) genes upon nutrient deprivation. However, little is known about their translational regulation. Here, we found that Dhh1, a DExD/H-box RNA helicase, is required for efficient translation of Atg1 and Atg13, two proteins essential for autophagy induction. Dhh1 directly associates with ATG1 and ATG13 mRNAs under nitrogen-starvation conditions. The structured regions shortly after the start codons of the two ATG mRNAs are necessary for their translational regulation by Dhh1. Both the RNA-binding ability and helicase activity of Dhh1 are indispensable to promote Atg1 translation and autophagy. Moreover, eukaryotic translation initiation factor 4E (EIF4E)-associated protein 1 (Eap1), a target of rapamycin (TOR)-regulated EIF4E binding protein, physically interacts with Dhh1 after nitrogen starvation and facilitates the translation of Atg1 and Atg13. These results suggest a model for how some ATG genes bypass the general translational suppression that occurs during nitrogen starvation to maintain a proper level of autophagy.
وصف الملف: electronic resource
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2
المؤلفون: Meiyan Jin, Sim Namkoong, Jun Hee Lee, Daniel J. Klionsky, Zhangyuan Yin, Zhiyuan Yao, Xu Liu
المصدر: PLoS Biology, Vol 17, Iss 4, p e3000219 (2019)
مصطلحات موضوعية: 0301 basic medicine, Atg1, Saccharomyces cerevisiae Proteins, QH301-705.5, Nitrogen, Autophagy-Related Proteins, Saccharomyces cerevisiae, Biology, Protein Serine-Threonine Kinases, General Biochemistry, Genetics and Molecular Biology, DEAD-box RNA Helicases, 03 medical and health sciences, 0302 clinical medicine, Eukaryotic translation, Proto-Oncogene Proteins, Translational regulation, Autophagy, Humans, Biology (General), Phosphorylation, Adaptor Proteins, Signal Transducing, General Immunology and Microbiology, General Neuroscience, EIF4E, Translation (biology), Autophagy-related protein 13, RNA Helicase A, Cell biology, 030104 developmental biology, HEK293 Cells, General Agricultural and Biological Sciences, Protein Kinases, 030217 neurology & neurosurgery, Protein Binding, Transcription Factors
الوصف: Macroautophagy (hereafter autophagy) is a well-conserved cellular process through which cytoplasmic components are delivered to the vacuole/lysosome for degradation and recycling. Studies have revealed the molecular mechanism of transcriptional regulation of autophagy-related (ATG) genes upon nutrient deprivation. However, little is known about their translational regulation. Here, we found that Dhh1, a DExD/H-box RNA helicase, is required for efficient translation of Atg1 and Atg13, two proteins essential for autophagy induction. Dhh1 directly associates with ATG1 and ATG13 mRNAs under nitrogen-starvation conditions. The structured regions shortly after the start codons of the two ATG mRNAs are necessary for their translational regulation by Dhh1. Both the RNA-binding ability and helicase activity of Dhh1 are indispensable to promote Atg1 translation and autophagy. Moreover, eukaryotic translation initiation factor 4E (EIF4E)-associated protein 1 (Eap1), a target of rapamycin (TOR)-regulated EIF4E binding protein, physically interacts with Dhh1 after nitrogen starvation and facilitates the translation of Atg1 and Atg13. These results suggest a model for how some ATG genes bypass the general translational suppression that occurs during nitrogen starvation to maintain a proper level of autophagy.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dd1c5e5e66707c2653959d95eef7f28Test
https://pubmed.ncbi.nlm.nih.gov/30973873Test
النص الكامل