دورية أكاديمية
Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells
| العنوان: | Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells |
|---|---|
| المؤلفون: | Zhu, Yanshan, Chew, Keng Yih, Wu, Melanie, Karawita, Anjana C., McCallum, Georgina, Steele, Lauren E., Yamamoto, Ayaho, Labzin, Larisa I., Yarlagadda, Tejasri, Khromykh, Alexander A., Wang, Xiaohui, Sng, Julian D.J., Stocks, Claudia J., Xia, Yao, Kollmann, Tobias R., Martino, David, Joensuu, Merja, Meunier, Frédéric A., Balistreri, Giuseppe, Bielefeldt-Ohmann, Helle, Bowen, Asha C., Kicic, Anthony, Sly, Peter D., Spann, Kirsten M., Short, Kirsty R. |
| المصدر: | PLoS Biology |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2022 |
| المجموعة: | Queensland University of Technology: QUT ePrints |
| الوصف: | Children typically experience more mild symptoms of Coronavirus Disease 2019 (COVID- 19) when compared to adults. There is a strong body of evidence that children are also less susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection with the ancestral viral isolate. However, the emergence of SARS-CoV-2 variants of concern (VOCs) has been associated with an increased number of pediatric infections. Whether this is the result of widespread adult vaccination or fundamental changes in the biology of SARS-CoV-2 remain to be determined. Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. This was associated with a heightened antiviral response to SARS-CoV-2 in the NECs of children. Importantly, the Delta variant also replicated to significantly lower titers in the NECs of children. This trend was markedly less pronounced in the case of Omicron. It is also striking to note that, at least in terms of viral RNA, Omicron replicated better in pediatric NECs compared to both Delta and the ancestral virus. Taken together, these data show that the nasal epithelium of children supports lower infection and replication of ancestral SARS-CoV-2, although this may be changing as the virus evolves. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Zhu, Yanshan, Chew, Keng Yih, Wu, Melanie, Karawita, Anjana C., McCallum, Georgina, Steele, Lauren E., Yamamoto, Ayaho, Labzin, Larisa I., Yarlagadda, Tejasri, Khromykh, Alexander A., Wang, Xiaohui, Sng, Julian D.J., Stocks, Claudia J., Xia, Yao, Kollmann, Tobias R., Martino, David, Joensuu, Merja, Meunier, Frédéric A., Balistreri, Giuseppe, Bielefeldt-Ohmann, Helle, Bowen, Asha C., Kicic, Anthony, Sly, Peter D., Spann, Kirsten M., & Short, Kirsty R. (2022) Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells. PLoS Biology, 20(8), Article number: e3001728.; https://eprints.qut.edu.au/241545Test/; Centre for Immunology and Infection Control; Faculty of Health; School of Biomedical Sciences |
| الإتاحة: | https://doi.org/10.1371/journal.pbio.3001728Test https://eprints.qut.edu.au/241545Test/ |
| حقوق: | © 2022 Zhu et al. ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: | edsbas.7AB510F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |