The seed coat-specific expression of a subtilisin-like gene, SCS1, from soybean
| العنوان: | The seed coat-specific expression of a subtilisin-like gene, SCS1, from soybean |
|---|---|
| المؤلفون: | Anthea K. Batchelor, Mark Gijzen, Ming Hu, Brian Miki, Douglas A. Johnson, LuAnne Bowman, S. Shea Miller, Hélène Labbé, Kim Boutilier |
| المصدر: | Planta 211 (2000) Planta, 211, 484-492 |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Seed coat, DNA, Complementary, Cellular differentiation, Molecular Sequence Data, Subtilisin-like gene, Plant Science, Biology, Gene Expression Regulation, Plant, Parenchyma, Gene expression, Genetics, Glycine (seed coat), Amino Acid Sequence, Northern blot, Cloning, Molecular, Gene, In Situ Hybridization, Base Sequence, Sequence Homology, Amino Acid, fungi, Subtilisin, Gene Expression Regulation, Developmental, food and beverages, RNA, Embryo, Parenchyma (thick walled), Biochemistry, RNA, Plant, Plant Research International, Soybeans |
| الوصف: | A seed coat-specific gene, SCS1 (Seed Coat Subtilisin 1), from soybean, Glycine max [L.] Merill, has been identified and studied. The gene belongs to a small family of genes with sequence similarity to the subtilisins, which are serine proteases. Northern blot analysis showed that SCS1 RNA accumulates to maximal levels in seed coats at 12 days post anthesis, preceding the final stages of seed coat differentiation. The SCS1 RNA was not found in other tissues including embryos, seed pods, flowers, stems, roots or leaves. In-situ hybridization studies confirmed the temporal pattern of expression observed by Northern blot analysis and further revealed a restricted pattern of RNA accumulation in thick-walled parenchyma cells of the seed coats. These cells are important in the apoplastic translocation of nutrients en route to the embryo from the vascular tissues. The tissue-specific subtilisin-like gene may be required for regulating the differentiation of the thick-walled parenchyma cells. |
| وصف الملف: | text/html; application/octet-stream |
| اللغة: | English |
| تدمد: | 0032-0935 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0f3f84dcdf562932f5a699466d03776Test https://doi.org/10.1007/s004250000320Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....f0f3f84dcdf562932f5a699466d03776 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00320935 |
|---|