Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems
| العنوان: | Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems |
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| المؤلفون: | Yongfang Wan, Paul Dupree, Huw Jones, Rowan A. C. Mitchell, Michael J. Ray, Peter R. Shewry, Keywan Hassani-Pak, Ambrose Andongabo, Theodora Tryfona, Angela Karp, C. Gritsch |
| المصدر: | Planta |
| بيانات النشر: | Springer Berlin Heidelberg, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | 0106 biological sciences, Willow, animal structures, LM10 antibody, macromolecular substances, Plant Science, 01 natural sciences, Lignin, Secondary xylem, Cell wall, 03 medical and health sciences, chemistry.chemical_compound, Cell Wall, Gene Expression Regulation, Plant, Botany, Genetics, RNA, Messenger, Cellulose, Genetic Association Studies, In Situ Hybridization, 030304 developmental biology, 0303 health sciences, biology, Plant Stems, fungi, technology, industry, and agriculture, Xylem, food and beverages, Salix, Salix purpurea, biology.organism_classification, Xylan, Hemicellulose, Tree stem transcriptome, chemistry, Original Article, Xylans, Phloem, Secondary cell wall, 010606 plant biology & botany |
| الوصف: | The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications. SCW development has been little studied in willow and it is not known how willow compares with model species, particularly the closely related genus Populus. To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development. A large increase in SCW deposition in the period 0–2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin. Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells. Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells. The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition. Electronic supplementary material The online version of this article (doi:10.1007/s00425-014-2034-1) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1432-2048 0032-0935 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8fefa96cbf7965d88e73f7cdef31a36Test http://europepmc.org/articles/PMC3997797Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d8fefa96cbf7965d88e73f7cdef31a36 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322048 00320935 |
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