Characterization of senescence-associated protease activities involved in the efficient protein remobilization during leaf senescence of winter oilseed rape
| العنوان: | Characterization of senescence-associated protease activities involved in the efficient protein remobilization during leaf senescence of winter oilseed rape |
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| المؤلفون: | Jean-Christophe Avice, Renier A. L. van der Hoorn, Marine Poret, Balakumaran Chandrasekar |
| المساهمون: | Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), French National Research Agency [ANR-11-BTBR-004], French Ministry of Research, PhD Doctoral School (EDNBISE, Ecole Doctorale Normande Biologie Integrative, Sante, Environnement), ERA-IB project 'PRODuCE', Max Planck Society, COST [CM1004], University of Oxford |
| المصدر: | Plant Science Plant Science, Elsevier, 2016, 246, pp.139-153. ⟨10.1016/j.plantsci.2016.02.011⟩ |
| بيانات النشر: | Elsevier BV, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Senescence, Proteasome Endopeptidase Complex, Proteases, Soluble proteins, Rapeseed, Ribulose-Bisphosphate Carboxylase, medicine.medical_treatment, Plant Science, Protein degradation, Biology, 01 natural sciences, Serine, 03 medical and health sciences, Tandem Mass Spectrometry, Genetics, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Protease activity, ComputingMilieux_MISCELLANEOUS, Plant Proteins, 2. Zero hunger, Cysteine proteases, Protease, Brassica napus, fungi, RuBisCO, food and beverages, General Medicine, Hydrogen-Ion Concentration, Senescence-associated proteases, Up-Regulation, Plant Leaves, 030104 developmental biology, Solubility, Proteasome, Biochemistry, Proteolysis, Seeds, Vacuoles, biology.protein, Nitrogen remobilization efficiency, Seasons, Agronomy and Crop Science, Chromatography, Liquid, Peptide Hydrolases, 010606 plant biology & botany |
| الوصف: | International audience; Oilseed rape (Brassica napus L.) is a crop plant characterized by a poor nitrogen (N) use efficiency that is mainly due to low N remobilization efficiency during the sequential leaf senescence of the vegetative stage. As a high leaf N remobilization efficiency was strongly linked to a high remobilization of proteins during leaf senescence of rapeseed, our objective was to identify senescence-associated protease activities implicated in the protein degradation. To reach this goal, leaf senescence processes and protease activities were investigated in a mature leaf becoming senescent in plants subjected to ample or low nitrate supply. The characterization of protease activities was performed by using in vitro analysis of RuBisCO degradation with or without inhibitors of specific protease classes followed by a protease activity profiling using activity-dependent probes. As expected, the mature leaf became senescent regardless of the nitrate treatment, and nitrate limitation enhanced the senescence processes associated with an enhanced degradation of soluble proteins. The characterization of protease activities revealed that: (i) aspartic proteases and the proteasome were active during senescence regardless of nitrate supply, and (ii) the activities of serine proteases and particularly cysteine proteases (Papain-like Cys proteases and vacuolar processing enzymes) increased when protein remobilization associated with senescence was accelerated by nitrate limitation. Short statement: Serine and particularly cysteine proteases (both PLCPs and VPEs) seem to play a crucial role in the efficient protein remobilization when leaf senescence of oilseed rape was accelerated by nitrate limitation. |
| وصف الملف: | application/pdf |
| تدمد: | 0168-9452 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37e669c817ae4468b032582c274323edTest https://doi.org/10.1016/j.plantsci.2016.02.011Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....37e669c817ae4468b032582c274323ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01689452 |
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