Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley
| العنوان: | Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley |
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| المؤلفون: | Renata Rucińska-Sobkowiak, Tomasz Wrzesinski, Władysław Polcyn, Lucyna Misztal, Autar K. Mattoo, Szymon Kubala, Agnieszka Bagniewska-Zadworna, Ewa Sobieszczuk-Nowicka |
| المصدر: | Plant Physiology. 178:654-671 |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Senescence, Programmed cell death, Time Factors, Light, Physiology, Glyoxylate cycle, Apoptosis, Plant Science, Mitochondrion, Models, Biological, 01 natural sciences, Tricarboxylate, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Autophagy, Genetics, Photosynthesis, Microautophagy, Chlorophyll fluorescence, Cell Nucleus, Chemistry, Gene Expression Profiling, Protoplasts, food and beverages, Hordeum, Articles, Darkness, Up-Regulation, Cell biology, Plant Leaves, 030104 developmental biology, Chlorophyll, Vacuoles, Carbohydrate Metabolism, 010606 plant biology & botany |
| الوصف: | Barley crop model was analyzed for early and late events during the dark-induced leaf senescence (DILS) as well as for deciphering critical time limit for reversal of the senescence process. Chlorophyll fluorescence vitality index Rfd was determined as the earliest parameter that correlated well with the cessation of photosynthesis prior to microautophagy symptoms, initiation of DNA degradation, and severalfold increase in the endonuclease BNUC1. DILS was found characterized by up-regulation of processes that enable recycling of degraded macromolecules and metabolites, including increased NH 4 + remobilization, gluconeogenesis, glycolysis, and partial up-regulation of glyoxylate and tricarboxylate acid cycles. The most evident differences in gene medleys between DILS and developmental senescence included hormone-activated signaling pathways, lipid catabolic processes, carbohydrate metabolic processes, low-affinity ammonia remobilization, and RNA methylation. The mega-autophagy symptoms were apparent much later, specifically on day 10 of DILS, when disruption of organelles—nucleus and mitochondria —became evident. Also, during this latter-stage programmed cell death processes, namely, shrinking of the protoplast, tonoplast interruption, and vacuole breakdown, chromatin condensation, more DNA fragmentation, and disintegration of the cell membrane were prominent. Reversal of DILS by re-exposure of the plants from dark to light was possible until but not later than day 7 of dark exposure and was accompanied by regained photosynthesis, increase in chlorophyll, and reversal of Rfd, despite activation of macro-autophagy-related genes. |
| تدمد: | 1532-2548 0032-0889 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3609ddaa47af8119cd6665e429b67a97Test https://doi.org/10.1104/pp.18.00516Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3609ddaa47af8119cd6665e429b67a97 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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