التفاصيل البيبلوغرافية
| العنوان: |
Rapid Identification of a Natural Knockout Allele of ARMADILLO REPEAT-CONTAINING KINESIN1 That Causes Root Hair Branching by Mapping-By-Sequencing. |
| المؤلفون: |
Rishmawi, Louai, Hequan Sun, Schneeberger, Korbinian, Hülskamp, Martin, Schrader, Andrea |
| المصدر: |
Plant Physiology; Nov2014, Vol. 166 Issue 3, p1280-1287, 8p |
| مصطلحات موضوعية: |
ALLELES in plants, PLANT genetics, GENETIC research, GENE knockout, KINESIN, ARABIDOPSIS thaliana, ROOT hairs (Botany), ROOT growth, BRANCHING (Botany) |
| مستخلص: |
In Arabidopsis (Arabidopsis thaliana), branched root hairs are an indicator of defects in root hair tip growth. Among 62 accessions, one accession (Heiligkreuztal2 [HKT2.4]) displayed branched root hairs, suggesting that this accession carries a mutation in a gene of importance for tip growth. We determined 200- to 300-kb mapping intervals using a mapping-by-sequencing approach of F2 pools from crossings of HKT2.4 with three different accessions. The intersection of these mapping intervals was 80 kb in size featuring not more than 36 HKT2.4-specific single nucleotide polymorphisms, only two of which changed the coding potential of genes. Among them, we identified the causative single nucleotide polymorphism changing a splicing site in ARMADILLO REPEAT-CONTAINING KINESIN1. The applied strategies have the potential to complement statistical methods in high-throughput phenotyping studies using different natural accessions to identify causative genes for distinct phenotypes represented by only one or a few accessions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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