Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe
| العنوان: | Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe |
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| المؤلفون: | K. C. Armstrong, Hoan T. Le, Brian Miki |
| المصدر: | Plant Molecular Biology Reporter. 7:150-158 |
| بيانات النشر: | Springer Science and Business Media LLC, 1989. |
| سنة النشر: | 1989 |
| مصطلحات موضوعية: | biology, food and beverages, Chromosome, Chromosomal translocation, Plant Science, Triticale, Molecular biology, genomic DNA, Somatic fusion, chemistry.chemical_compound, chemistry, Botany, biology.protein, Molecular Biology, DNA, Satellite chromosome, Peroxidase |
| الوصف: | Total DNA was extracted formSecale cereale L. cv. ‘Petkus’ and labeled with biotin-11-dUPT. Labeled rye DNA and non-labeled wheat DNA in a mixture of 1∶1 were used as a probe on chromosome preparations of Welsh triticale and Kavkaz wheat, a wheat translocation stock. Hybridization of denatured probe and chromosomes took place overnight at 37°C in the presence of 10% (w/v) dextran sulfate, 50% (v/v) formamide, 10 mM PIPES, 0.1 mMEDTA and 0.3 M NaC1. Biotin-labeled rye DNA was detected using streptavidin-horseradish peroxidase conjugate. Staining was made with diaminobenzidine tetrahydrochloride and hydrogen peroxide. Observations made on Giesma counter-stained slides indicated that the rye chromosomes in Welsh triticale and the two short arms of a pair of satellite chromosomes (1RS) in Kavkaz wheat were preferentially labeled. Hybridization signals were seen as dark brown to bluish black in color. |
| تدمد: | 1572-9818 0735-9640 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ae5998d0b0e54dcd305c6d687bb76ccTest https://doi.org/10.1007/bf02669631Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ae5998d0b0e54dcd305c6d687bb76cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15729818 07359640 |
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