دورية
Mitochondrial malate dehydrogenase from watermelon: sequence of cDNA clones and primary structure of the higher-plant precursor protein
| العنوان: | Mitochondrial malate dehydrogenase from watermelon: sequence of cDNA clones and primary structure of the higher-plant precursor protein |
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| المؤلفون: | Gietl, Christine, Lehnerer, Michael, Olsen, Ole |
| المصدر: | Plant Molecular Biology; June 1990, Vol. 14 Issue: 6 p1019-1030, 12p |
| مستخلص: | The isolation and sequence of a cDNA clone encoding the complete mitochondrial malate dehydrogenase (mMDH) of watermelon cotyledons is presented. Taking advantage of the polymerase chain reaction technology partial cDNA clones from the central part, the 3' part and the 5' part of the mRNA were obtained with oligonucleotides based on directly determined amino acid sequences. Subsequently, two complete cDNA clones for mMDH were synthesized with a sense primer corresponding to the nucleotide sequence of the amino terminal end of pre-mMDH and two antisense primers corresponding to the major alternative adenylation sites found in the mRNA. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 01674412 15735028 |
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| DOI: | 10.1007/BF00019398 |