التفاصيل البيبلوغرافية
| العنوان: |
Complete Genome Sequence Analysis of Guangdong Isolates of Cymbidium Mosaic Virus. |
| المؤلفون: |
Yonglu WEI1, Rui REN1, Jie GAO1, Weiping LIU2, Qi XIE1, Genfa ZHU1, Fengxi YANG1 fengxi_wei@sina.com |
| المصدر: |
Plant Diseases & Pests. Jun2022, Vol. 13 Issue 3, p15-20. 6p. |
| مصطلحات موضوعية: |
WHOLE genome sequencing, SEQUENCE analysis, MOSAIC viruses, GENETIC drift, GENETIC engineering, GENOMES, REVERSE transcriptase polymerase chain reaction |
| مصطلحات جغرافية: |
GUANGDONG Sheng (China), NANJING (Jiangsu Sheng, China), TAIWAN |
| مستخلص: |
[Objectives] The paper was to explore the genetic information and evolution of CymMV, and to provide an important scientific basis for monitoring and early warning of orchid virus disease and anti-virus genetic engineering of orchid in Guangdong Province. [Methods] RT-PCR and DASELISA were used to detect and identify CymMV from leaves with suspected virus disease of Cymbidium sinense collected from Guangzhou area. The genome sequence assembly, annotation, phylogeny and selection pressure analysis of CymMV isolates were performed with related molecular biology software. [Results] Two CymMV isolates (GZV013 and ZC29) were found in Guangdong Province for the first time in this study. The genome of both GZV013 and ZC29 were 6 227nt in length, encoding 5 functional proteins. The similarity analysis of the full sequence showed that the nucleotide sequence identity of GZV013 and Taiwan isolate M2 was 97.03% and that of ZC29 and Nanjing isolate NJ-1 was 97.11%. The complete genome sequence identity among CymMV isolates ranged from 86.85% to 98.31 %, and the differentiation of diverse populations was closely related to host species and geographical isolation. Each region of CymMV genome was affected by negative selection and conformed to the neutral evolution model. The genes encoding RdRp, TGB1 and TGB2 had the highest mutation rates in the genome. [ Conclusions] GZV013 was most closely related to Taiwan isolate M2, and ZC29 was most closely related to Nanjing isolate NJ-1, belonging to the same branch of a family. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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