المؤلفون: Masayuki Murai, Toshiyuki Komori, Toshihiko Komari, Masahiko Maekawa, Noriko Ishikawa, Yukoh Hiei, Emily Gichuhi, Yuji Ishida, Eiko Himi, Masakazu Kashihara

المصدر: Plant Breeding. 140:409-418

مصطلحات موضوعية: Oryza sativa, biology, Pseudo-response regulator, Botany, Genetics, Introgression, Oryza longistaminata, Plant Science, Allele, biology.organism_classification, Agronomy and Crop Science, High yielding

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::a85e32fb871ea38a8f01d3b066ef8edeTest
https://doi.org/10.1111/pbr.12910Test

المؤلفون: Toshihiko Komari, Toshiyuki Komori, Maiko Miyata, Yukoh Hiei, Masahiro Yano, Jun Ueki, Toshio Yamamoto, Naoto Nitta

المصدر: Plant Breeding. 128:568-575

مصطلحات موضوعية: Genetics, Oryza sativa, biology, Sequence analysis, Transgene, food and beverages, Locus (genetics), Plant Science, biology.organism_classification, Japonica, Complementary DNA, Allele, Agronomy and Crop Science, Gene

الوصف: The stub-spreading trait, also designated as ‘tiller angle’, is one of the components that determine the plant type. In rice, the trait is quite important because of its close relationship to the yield performance. The Spk(t) gene is a major determinant of the trait in the cross of ‘Kasalath’ (indica) and ‘Nipponbare’ (japonica). In this study, the Spk(t) gene was isolated by a map-based cloning strategy. Sequence analysis of the cDNA clones from the locus revealed that the Spk(t) transcripts and the spk(t) transcripts encode identical 259-aa proteins of unknown function; however, the structure of the 3′-untranslated region of each allele is quite different. Additional transgenic experiments in rice demonstrated that the difference is caused by a single-nucleotide polymorphism at the 3′-splicing site specific to the Spk(t) allele which thereby plays a critical role in the phenotypic expression. In addition to revealing the molecular mechanism underlying the allele differentiation at the Spk(t) locus, the information presented here should be useful for breeding of this trait in rice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5b7770da632227f372a81d2947db57fcTest
https://doi.org/10.1111/j.1439-0523.2009.01639.xTest

المؤلفون: N. Nitta, Toshiyuki Komori

المصدر: Plant Breeding. 123:549-553

مصطلحات موضوعية: Genetics, Oryza sativa, Software maintainer, Cytoplasmic male sterility, food and beverages, Plant Science, Biology, biology.organism_classification, Japonica, law.invention, law, Genetic marker, Agronomy and Crop Science, Gene, Polymerase chain reaction, Hybrid

الوصف: Cytoplasmic male sterility (CMS) by the cms-bo cytoplasm and its restoration by the nuclear restorer gene, Rf-1, are used for seed production of japonica hybrid rice varieties. To produce pure hybrid seeds, a prerequisite is to properly manage the seed purity of parental lines, especially CMS lines. In this study, three dominant polymerase chain reaction (PCR)-based markers (M1, M2 and M3) were developed to detect mutual contamination in seed batches of CMS lines, maintainer lines, restorer lines and hybrids. M1 detected the mitochondrial sequence that was present in the cytoplasm of common japonica varieties and absent in the cms-bo cytoplasm. M2 and M3 detected the chromosomal sequence related to the Rf-1 allele in restorer lines and the rf-1 allele in common japonica varieties, respectively. By the strategic use of these markers, japonica hybrids and their parental lines could be efficiently distinguished from each other. Furthermore, sensitivity tests for the three markers with a series of crude DNA samples prepared from polished grains demonstrated that these markers could detect one contaminating grain among 500 or 1000 grains. Therefore, the bulk PCR analyses with the markers developed here probably make it possible to control the seed purity of japonica hybrids properly by selecting appropriate seed batches of their parental lines quickly and efficiently.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::4bcf56b0e3e048a0713bdcc4bf55fe2dTest
https://doi.org/10.1111/j.1439-0523.2004.01029.xTest