A simple method to control the seed purity of japonica hybrid rice varieties using PCR-based markers
| العنوان: | A simple method to control the seed purity of japonica hybrid rice varieties using PCR-based markers |
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| المؤلفون: | N. Nitta, Toshiyuki Komori |
| المصدر: | Plant Breeding. 123:549-553 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Genetics, Oryza sativa, Software maintainer, Cytoplasmic male sterility, food and beverages, Plant Science, Biology, biology.organism_classification, Japonica, law.invention, law, Genetic marker, Agronomy and Crop Science, Gene, Polymerase chain reaction, Hybrid |
| الوصف: | Cytoplasmic male sterility (CMS) by the cms-bo cytoplasm and its restoration by the nuclear restorer gene, Rf-1, are used for seed production of japonica hybrid rice varieties. To produce pure hybrid seeds, a prerequisite is to properly manage the seed purity of parental lines, especially CMS lines. In this study, three dominant polymerase chain reaction (PCR)-based markers (M1, M2 and M3) were developed to detect mutual contamination in seed batches of CMS lines, maintainer lines, restorer lines and hybrids. M1 detected the mitochondrial sequence that was present in the cytoplasm of common japonica varieties and absent in the cms-bo cytoplasm. M2 and M3 detected the chromosomal sequence related to the Rf-1 allele in restorer lines and the rf-1 allele in common japonica varieties, respectively. By the strategic use of these markers, japonica hybrids and their parental lines could be efficiently distinguished from each other. Furthermore, sensitivity tests for the three markers with a series of crude DNA samples prepared from polished grains demonstrated that these markers could detect one contaminating grain among 500 or 1000 grains. Therefore, the bulk PCR analyses with the markers developed here probably make it possible to control the seed purity of japonica hybrids properly by selecting appropriate seed batches of their parental lines quickly and efficiently. |
| تدمد: | 1439-0523 0179-9541 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::4bcf56b0e3e048a0713bdcc4bf55fe2dTest https://doi.org/10.1111/j.1439-0523.2004.01029.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........4bcf56b0e3e048a0713bdcc4bf55fe2d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390523 01799541 |
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