التفاصيل البيبلوغرافية
| العنوان: |
Accumulation Pattern of IgG Antibodies and Fab Fragments in Transgenic Arabidopsis thaliana Plants. |
| المؤلفون: |
Wilde, Chris De, Rycke, Riet De, Beeckman, Tom, Neve, Myriam De, Montagu, Marc Van, Engler, Gilbert, Depicker, Ann |
| المصدر: |
Plant & Cell Physiology; Jun1998, Vol. 39 Issue 6, p639-646, 8p |
| مصطلحات موضوعية: |
ARABIDOPSIS, IMMUNOGLOBULIN G, TRANSGENIC plants, PLANT genetic engineering, PLANT transpiration, RECOMBINANT antibodies, BIOLOGICAL transport, PLANTS |
| مستخلص: |
For the further optimization of antibody expression in plants, it is essential to determine the final accumulation sites of plant-made antibodies. Previously, we have shown that, upon secretion, IgG antibodies and Fab fragments can be detected in the intercellular spaces of leaf mesophyil cells of transgenic Arabidopsis thaliana plants. However, immunofluorescence microscopy showed that this is probably not their final accumulation site. In leaves, IgG and Fabfragments accumulate also at the interior side of the epidermal cell layers and in xylem vessels. These accumulation sites correspond with the leaf regions where water of the transpiration stream is entering a space impermeable to the proteins or where water is evaporating. In roots, plant-made Fab fragments accumulate in intercellular spaces of cortex cells, in the cytoplasm of pericycle and, to a lesser extent, endodermis cells, and in cells of the vascular cylinder. In other words, antibody accumulation occurs at the sites where water passes on its radial pathway towards and within the vascular bundle. Taken together, our results suggest that, upon secretion of plant-made antibodies or Fab fragments, a large proportion of these proteins are transported in the apoplast of A. thaliana, possibly by the water flow in the transpiration stream. [ABSTRACT FROM AUTHOR] |
|
Copyright of Plant & Cell Physiology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder