A simplified procedure to trace triglyceride-rich lipoprotein metabolism in vivo
| العنوان: | A simplified procedure to trace triglyceride-rich lipoprotein metabolism in vivo |
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| المؤلفون: | Sander Kooijman, Zhixiong Ying, Tamer Coskun, Patrick C.N. Rensen, Mariëtte R. Boon |
| المصدر: | Physiological Reports Physiological Reports, 9(8). WILEY Physiological Reports, Vol 9, Iss 8, Pp n/a-n/a (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Very low-density lipoprotein, Physiology, Sonication, Lipoproteins, plasma triglyceride metabolism, Fractionation, 030204 cardiovascular system & hematology, Tritium, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Physiology (medical), Glycerol, Lipolysis, QP1-981, Animals, Tissue Distribution, Carbon Radioisotopes, Triglycerides, lipoprotein metabolism, Chromatography, lipoprotein kinetics, Metabolism, Original Articles, Mice, Inbred C57BL, Cholesterol, chemistry, Adipose Tissue, Density gradient ultracentrifugation, Original Article, VLDL, 030217 neurology & neurosurgery, Triolein, Lipoprotein |
| الوصف: | Glycerol tri[H-3]oleate and [C-14]cholesteryl oleate double-labeled triglyceride-rich lipoprotein (TRL)-like particles are a well-established tool to trace the effect of lipid-modulating interventions on TRL metabolism. The routine generation of these particles involves sonication of a lipid mixture and subsequent fractionation of resulting particles into populations of different average size through density gradient ultracentrifugation. Here, we describe a simplified and more time-efficient procedure for preparing TRL-like particles without the need of fractionation. The simplified procedure shortened the preparation of particles from over 4 h to less than 2 h and generated particles with a higher yield, although with a smaller average size and more heterogeneous size distribution. In C57Bl/6J mice housed at thermoneutrality (30 degrees C), the two preparations showed highly comparable plasma clearance and organ distribution of glycerol tri[H-3]oleate-derived [H-3]oleate and [C-14]cholesteryl oleate, as measures of lipolysis and core remnant uptake, respectively. Upon a cold challenge (14 degrees C), plasma clearance was accelerated due to enhanced uptake of glycerol tri[H-3]oleate-derived [H-3]oleate by brown adipose tissue. The simplified procedure resulted in a modestly increased particle uptake by the spleen, while uptake by other organs was comparable between the two preparations. In conclusion, the simplified procedure accelerates the preparation of TRL-like particles for tracing in vivo TRL metabolism. We anticipate that this time-efficient procedure will be useful for incorporation of PET-traceable lipids to obtain more insight into human lipoprotein metabolism. |
| وصف الملف: | application/pdf |
| تدمد: | 2051-817X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbdf002c4c8fdbeb34ef89b0c7e7ef2eTest https://pubmed.ncbi.nlm.nih.gov/33945228Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fbdf002c4c8fdbeb34ef89b0c7e7ef2e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2051817X |
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