دورية أكاديمية
Quantitative Analysis of Single-Molecule Force Spectroscopy on Folded Chromatin Fibers
| العنوان: | Quantitative Analysis of Single-Molecule Force Spectroscopy on Folded Chromatin Fibers |
|---|---|
| المؤلفون: | Meng, He, Andresen, Kurt, Van Noort, John |
| المصدر: | Physics and Astronomy Faculty Publications |
| بيانات النشر: | The Cupola: Scholarship at Gettysburg College |
| سنة النشر: | 2015 |
| المجموعة: | The Cupola - Scholarship at Gettysburg College |
| مصطلحات موضوعية: | Gene regulation, Chromatin and Epigenetics, Biological and Chemical Physics |
| الوصف: | Single-molecule techniques allow for picoNewton manipulation and nanometer accuracy measurements of single chromatin fibers. However, the complexity of the data, the heterogeneity of the composition of individual fibers and the relatively large fluctuations in extension of the fibers complicate a structural interpretation of such force-extension curves. Here we introduce a statistical mechanics model that quantitatively describes the extension of individual fibers in response to force on a per nucleosome basis. Four nucleosome conformations can be distinguished when pulling a chromatin fiber apart. A novel, transient conformation is introduced that coexists with single wrapped nucleosomes between 3 and 7 pN. Comparison of force-extension curves between single nucleosomes and chromatin fibers shows that embedding nucleosomes in a fiber stabilizes the nucleosome by 10 kBT. Chromatin fibers with 20- and 50-bp linker DNA follow a different unfolding pathway. These results have implications for accessibility of DNA in fully folded and partially unwrapped chromatin fibers and are vital for understanding force unfolding experiments on nucleosome arrays. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://cupola.gettysburg.edu/physfac/124Test; https://cupola.gettysburg.edu/cgi/viewcontent.cgi?article=1131&context=physfacTest |
| الإتاحة: | https://cupola.gettysburg.edu/physfac/124Test https://cupola.gettysburg.edu/cgi/viewcontent.cgi?article=1131&context=physfacTest |
| رقم الانضمام: | edsbas.B1EAA529 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |