Apoptosis in glioma cells treated with PDT
| العنوان: | Apoptosis in glioma cells treated with PDT |
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| المؤلفون: | Luis Fernando Tirapelli, Harley Francisco de Oliveira, Carlos Gilberto Carlotti Júnior, Marcelo Morgueti, Fermino Sanches Lizarte Neto, Daniela Pretti da Cunha Tirapelli, Juliana Carvalho Ferreira, Vanderlei Salvador Bagnato, Fernanda Maris Peria |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, NEOPLASIAS CEREBRAIS (TRATAMENTO), Photosensitizing Agents, business.industry, Cell Survival, Biomedical Engineering, Cell Culture Techniques, Apoptosis, Photosensitizing Agent, Glioma, medicine.disease, Photochemotherapy, Cell culture, Cell Line, Tumor, Cancer research, Medicine, Humans, Radiology, Nuclear Medicine and imaging, business, Cell survival, Median survival, Glioblastoma |
| الوصف: | Despite significant advances in neurosurgical techniques, the median survival time of patients with glioblastoma has improved little over the past 50 years and remains less than one year. Photodynamic therapy (PDT) is presently established as a widely accepted modality for the treatment of a variety of solid tumors.This study evaluated the effect of PDT-Photogem(®) on five glioma cell lines (U87, U138, U251, U343, and T98G).The experiments were carried out in 25-cm(3) flasks with different groups of cells seeded at a density of 1 × 10(5) cells per flask. After 3 h, the medium was removed, and the cells were incubated for 4 h with Photogem (5 μg/mL). After the incubation time, the photosensitizer-containing medium was removed and the cells were irradiated with LED (630 nm, 25 mW/cm(2), 25 J/cm(2)) devices for 17 min. For the final steps of the PDT, the cells were returned to the incubator and kept at 37°C with 5% CO(2) for 24 h, the cell viability assay was assessed using the trypan blue method, and the expression of Caspase 3 mRNA levels was assessed by real-time quantitative PCR.Upon PDT-Photogem(®) treatment, viable cells, as evaluated by the trypan blue dye-exclusion method, decreased in two cell lines (U87 and U138) but not in the other three. Apoptosis, as assessed by the expression of caspase-3 mRNA levels, was at least partly involved in the death mechanism of the cell lines.Collectively, our results indicated that PDT-Photogem(®) can act in glioma cells, thus encouraging new experiments in this field. |
| تدمد: | 1557-8550 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1877b63cf5376fe7032af6cbdb5e6b4bTest https://pubmed.ncbi.nlm.nih.gov/21254884Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1877b63cf5376fe7032af6cbdb5e6b4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578550 |
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