التفاصيل البيبلوغرافية
| العنوان: |
Whole Transcription Profile of Responders to Anti-TNF Drugs in Pediatric Inflammatory Bowel Disease. |
| المؤلفون: |
Salvador-Martín, Sara, Kaczmarczyk, Bartosz, Álvarez, Rebeca, Navas-López, Víctor Manuel, Gallego-Fernández, Carmen, Moreno-Álvarez, Ana, Solar-Boga, Alfonso, Sánchez, Cesar, Tolin, Mar, Velasco, Marta, Muñoz-Codoceo, Rosana, Rodriguez-Martinez, Alejandro, Vayo, Concepción A., Bossacoma, Ferrán, Pujol-Muncunill, Gemma, Fobelo, María J., Millán-Jiménez, Antonio, Magallares, Lorena, Martínez-Ojinaga, Eva, Loverdos, Inés |
| المصدر: |
Pharmaceutics; Jan2021, Vol. 13 Issue 1, p77, 1p |
| مصطلحات موضوعية: |
INFLAMMATORY bowel diseases, PHARMACOGENOMICS, GENE expression profiling, CHILD patients, DRUGS, NUCLEOTIDE sequence |
| مستخلص: |
Background: Up to 30% of patients with pediatric inflammatory bowel disease (IBD) do not respond to anti-Tumor Necrosis Factor (anti-TNF) therapy. The aim of this study was to identify pharmacogenomic markers that predict early response to anti-TNF drugs in pediatric patients with IBD. Methods: An observational, longitudinal, prospective cohort study was conducted. The study population comprised 38 patients with IBD aged < 18 years who started treatment with infliximab or adalimumab (29 responders and nine non-responders). Whole gene expression profiles from total RNA isolated from whole blood samples of six responders and six non-responders taken before administration of the biologic and after two weeks of therapy were analyzed using next-generation RNA sequencing. The expression of six selected genes was measured for purposes of validation in all of the 38 patients recruited using qPCR. Results: Genes were differentially expressed in non-responders and responders (32 before initiation of treatment and 44 after two weeks, Log2FC (Fold change) >0.6 or <−0.6 and p value < 0.05). After validation, FCGR1A, FCGR1B, and GBP1 were overexpressed in non-responders two weeks after initiation of anti-TNF treatment (Log2FC 1.05, 1.21, and 1.08, respectively, p value < 0.05). Conclusion: Expression of the FCGR1A, FCGR1B, and GBP1 genes is a pharmacogenomic biomarker of early response to anti-TNF agents in pediatric IBD. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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