دورية أكاديمية
A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets
| العنوان: | A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets |
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| المؤلفون: | Tikhonova, M. A., Maslov, N. A., Bashirzade, A. A., Nehoroshev, E. V., Babchenko, V. Y., Chizhova, N. D., Tsibulskaya, E. O., Akopyan, A. A., Markova, E. V., Yang, Y. -L., Lu, K. -T., Kalueff, A. V., Aftanas, L. I., Amstislavskaya, T. G. |
| المصدر: | Pharmaceutics |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Ural Federal University (URFU): ELAR / Уральский федеральный университет: электронный архив УрФУ |
| مصطلحات موضوعية: | ANIMAL MODEL, BEHAVIOR, LASER, NEURODEGENERATION, NEUROINFLAMMATION, NEUROREPAIR, TRAUMATIC BRAIN INJURY, ZEBRAFISH, BRAIN DERIVED NEUROTROPHIC FACTOR, CELL PROTEIN, HYDROCORTISONE, HYPOXIA INDUCIBLE FACTOR 1ALPHA, IONIZED CALCIUM BINDING ADAPTER MOLECULE 1 PROTEIN, NEURON SPECIFIC NUCLEAR PROTEIN, UNCLASSIFIED DRUG, ADULT, ANIMAL CELL, ANIMAL EXPERIMENT, ANIMAL TISSUE, ANXIETY DISORDER, ARTICLE, BEHAVIOR ASSESSMENT, CELL ACTIVATION, CELL DAMAGE, CONSTRUCT VALIDITY, CONTROLLED STUDY, CONVALESCENCE, FACE VALIDITY, FEMALE, HYPOLOCOMOTION |
| الوصف: | Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening. © 2022 by the authors. ; Saint Petersburg State University, SPbU: 73026081; Russian Science Foundation, RSF: 20-65-46006 ; We thank Alisa S. Belova for technical support in experimental manipulations and cortisol assay. We also thank Anatoly A. Maslov for the idea of using laser radiation to introduce brain damage. A.V.K. lab is supported by St. Peterburg State University funds (Pure ID 73026081). ; This study was funded by Russian Science Foundation (grant No. 20-65-46006). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-85-13-73883-2 85-13-73883-2 |
| تدمد: | 19994923 |
| العلاقة: | A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets / M. A. Tikhonova, N. A. Maslov, A. A. Bashirzade et al. // Pharmaceutics. — 2022. — Vol. 14. — Iss. 8. — 1751.; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137388324&doi=10.3390%2fpharmaceutics14081751&partnerID=40&md5=bda19df98ae2372fe6a731d67450bbccTest; http://elar.urfu.ru/handle/10995/118086Test; 85137388324; 000845798700001 |
| DOI: | 10.3390/pharmaceutics14081751 |
| الإتاحة: | https://doi.org/10.3390/pharmaceutics14081751Test http://elar.urfu.ru/handle/10995/118086Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.33082C94 |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9788513738832 8513738832 |
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| تدمد: | 19994923 |
| DOI: | 10.3390/pharmaceutics14081751 |