Human Pancreatic Polypeptide in a Phospholipid-Based Micellar Formulation
| العنوان: | Human Pancreatic Polypeptide in a Phospholipid-Based Micellar Formulation |
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| المؤلفون: | Amrita Banerjee, Hayat Onyuksel |
| المصدر: | Pharmaceutical Research. 29:1698-1711 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Protein Denaturation, Circular dichroism, Time Factors, Light, Chemistry, Pharmaceutical, Drug Compounding, Phospholipid, Pharmaceutical Science, Sodium Chloride, Pancreatic Polypeptide, Micelle, Article, Polyethylene Glycols, Neuroblastoma, chemistry.chemical_compound, Drug Stability, Cell Line, Tumor, Scattering radiation, Cyclic AMP, Humans, Hypoglycemic Agents, Nanotechnology, Scattering, Radiation, Technology, Pharmaceutical, Pancreatic polypeptide, Pharmacology (medical), Particle Size, Protein Precursors, Chromatography, High Pressure Liquid, Micelles, Pharmacology, Drug Carriers, Aqueous solution, Circular Dichroism, Phosphatidylethanolamines, Colforsin, Organic Chemistry, Hydrogen-Ion Concentration, Human pancreatic polypeptide, Receptors, Neuropeptide Y, Freeze Drying, Spectrometry, Fluorescence, chemistry, Biochemistry, Nanoparticles, Molecular Medicine, Drug carrier, Biotechnology |
| الوصف: | Pancreatic polypeptide (PP) has important glucoregulatory functions and thereby holds significance in the treatment of diabetes and obesity. However, short plasma half-life and aggregation propensity of PP in aqueous solution, limits its therapeutic application. To address these issues, we prepared and characterized a formulation of PP in sterically stabilized micelles (SSM) that protects and stabilizes PP in its active conformation.PP-SSM was prepared by incubating PP with SSM dispersion in buffer. Peptide-micelle association and freeze-drying efficacy of the formulation was characterized in phosphate buffers with or without sodium chloride using dynamic light scattering, fluorescence spectroscopy and circular dichroism. The degradation kinetics of PP-SSM in presence of proteolytic enzyme was determined using HPLC and bioactivity of the formulation was evaluated by in vitro cAMP inhibition study.PP self-associated with SSM and this interaction was influenced by presence/absence of sodium chloride in the buffer. The formulation was effectively lyophilized, demonstrating feasibility for its long-term storage. The stability of peptide against proteolytic degradation was significantly improved and PP in SSM retained its bioactivity in vitro.Self-association of PP with phospholipid micelles addressed the delivery issues of the peptide. This nanomedicine should be further developed for the treatment of diabetes. |
| تدمد: | 1573-904X 0724-8741 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bb83b15ac6beb2e84a1266f4fef8b79Test https://doi.org/10.1007/s11095-012-0718-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0bb83b15ac6beb2e84a1266f4fef8b79 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1573904X 07248741 |
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