التفاصيل البيبلوغرافية
| العنوان: |
用 HPLC 法同时测定扶正平消胶囊中龙胆苦苷和迷迭香酸的含量. |
| العنوان البديل: |
Simultaneous determination of gentiopicroside and rosmarinic acid in Fuzhengpingxiao capsules by HPLC method. |
| المؤلفون: |
王 慧1, 李 洋1, 周燕妮1, 费 扬1, 万贺临1, 张国庆1 |
| المصدر: |
Pharmaceutial Care & Research. Dec2015, Vol. 15 Issue 6, p436-438. 3p. |
| الملخص (بالإنجليزية): |
Objective ： To establish a HPLC method for simultaneous determination of gentiopicroside and rosmarinic acid in Fuzhengpingxiao capsules . Methods :Agilent Eclipse XDB-C18 column (250 mm × 4 .6 mm，5 pm ) was used for separation . Mobile phase was composed of 0 .1% formic acid (A )-acetonitrile(B) in gradient elution at a flow rate of 1 .0 ml/min:0-5 min 88% A，5-10 min 88%-82% A ,10-15min 82%-70% A，15-20 min 70%-60% A . The column temperature was 25 °C and the detection wavelength was 280 nm .Results ：The calibration curves of gentiopicroside and rosmarinic acid showed good linearity in the ranges of 6.75-431.6 μg/ml(r=1.000) and 3.16-201.8 μg/ml(r=1.000) , respectively . The results of intra-day and interday precisions were both within 2% (n = 3 ). The average additional recovery rates were (100.89 ± 2.25)% and (98.19 ± 1.13)% (n= 6)，respectively. Conclusion: The HPLC method proves to be simple，accurate and reproducible. It could be used for quality control of gentiopicroside and rosmarinic acid in the preparation of Fuzhengpingxiao capsules. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：建立 HPLC 法同时测定扶正平消胶囊中龙胆苦苷和迷迭香酸的含量。方法：采用Agilent Eclipse XDB-C18 色谱柱 (250 mm×4.6 mm，5 μm)，以0.1%甲酸(A)-乙腊(B)为流动相进行梯度洗脱，0〜5 min 88% A，5〜10 min 88%〜82% A，10〜15 min 82%〜70% A，15〜20 min 70%〜60% A ;流速 1 .0 ml/min ;柱温 25 °C; 检测波长 280 nm。结果： 龙胆苦苷在6 .75〜431.6 μg/ml(r=1.000)，迷迭香酸在 3.16〜201.8 μg/ml 范围内呈良好线性（r=1.000)。日内和日间精 密度均<2%(n=3)。平均加样回收率分别为(100.89×2.25)%和(98.19×1.13)% (n=6)。结论:该方法简便、结果准确、 重复性好，可用于扶正平消胶囊中龙胆苦苷和迷迭香酸的质量控制。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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