التفاصيل البيبلوغرافية
| العنوان: |
基于网络药理学探讨益气活血方抗肝纤维化作用的活性成分及其分子 机制研究 (Chinese) |
| العنوان البديل: |
Investigation on the active components and molecular mechanisms involved in the Yiqi Huoxue decoction against liver fibrosis based on network pharmacology. (English) |
| المؤلفون: |
杨 提, 曾婷婷, 张卉青, 叶琳岚, 蔺婷婷, 丁 楠 |
| المصدر: |
Pharmaceutial Care & Research; Dec2021, Vol. 21 Issue 6, p412-423, 8p |
| مصطلحات موضوعية: |
MITOGEN-activated protein kinases, PROTEIN-protein interactions, MEDICAL databases, SMALL molecules, CHINESE medicine, RECEPTOR for advanced glycation end products (RAGE), SERINE/THREONINE kinases |
| الملخص (بالإنجليزية): |
Objective: To predict and explore the active components and potential mechanisms of the Yiqi Huoxue decoc - tion against liver fibrosis by network pharmacology. Methods: The active components and related potential targets of the Yiqi Huoxue decoction were screened out by the Traditional Chinese M edicine Systems Pharmacology Database and Analysis Plat - form (TCMSP) and the Traditional Chinese Medicine Integrated Database (TCM ID). The GeneCards database was used to find out related targets of liver fibrosis. Based on the matching results of potential targets of Yiqi Huoxue decoction and related liver fibrosis targets, the String platfo rm was used to analyze protein interactions and construct protein-protein interaction network. Core targets were screened by topology analysis. Cytoscape 3.7.2 was applied to construct the active co m po ne nt -live r fibrosistarget network of Yiqi Huoxue decoction, and key active components were screened out by topological analysis. Finally gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed for common targets of drugs and diseases through R language. Results: The core active components of Yiqi Huoxue decoction against liver fibrosis were mainly quercetin Juteolin, kaempferol, naringin, tanshinone II a yetc. The core targets included signal transducer and activator of transcription-3 (STAT3), tumor necrosis facto (TNF-«), serine/threonine protein kinase-1 (AKT1), pro to-oncogene (JUN), TP53, 妝. These targets were mainly involved in the biological processes, such as the response process of nutrients and lipopolysaccharide, oxidative stress, steroid metabolism Jipopolysaccharide, reactive oxygen species, and the regulation of small molecule metabolism. They were mainly concentrated in phosphatidylinositol-4,5-biphosphate 3-kinase-protein kinase B (Pl3K~Akt) signaling pathway, fluid shear stress and atherosclerosis, advanced glycation and products-receptor of AGE (AGE-RAGE) signaling pathway in diabetic complications, mitogen-activated protein kinase (M APK) signaling pathway, TNF-« signaling pathway, interleukin-17 (IL-17) signaling pathway and other signaling pathways. Conclusion: Yiqi Huoxue decoction has such features of multi-components, multi-targets and multrpathways in anti-hepatic fibrosis. This study has provided a solid theoretical basis and reference for followup experimental study on the anti-hepatic fibrosis mechanism of Yiqi Huoxue decoction. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：通过网络药理学方法预测和探讨益气活血方抗肝纤维化的活性成分及其潜在的作用机制。方法：采用中 药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)、中医药综合数据库(Traditional Chinese Medicine Integrated Database, TCMID)筛选出益气活血方的活性成分和相 关的潜在靶点;利用GeneCards数据库检索肝纤维化相关靶点;基于益气活血方潜在靶点与肝纤维化相关靶点的匹配结果, 利 用String平台进行蛋白质相互作用分析, 构建蛋白质相互作用网络, 拓扑分析筛选核心靶点;利用Cytoscape 3 .7 .2构建益气 活血方活性成分-肝纤维化-靶点网络, 拓扑分析筛选出关键活性成分;再利用R语言软件对药物与疾病共有靶点分别进行基 因本体(gene ontology, G0)功能、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)通路富集分 析。结果：益气活血方抗肝纤维化的核心活性成分为榊皮素、木犀草素、山奈酚、柚皮素、丹参酮Ua等。核心靶点有转录激 活因子一 3(signal transducer and activator of transcript ion -3, STAT3)、月中 瘤坏死因子一 a(tuinor necrosis factorp, TNFp)、丝氨 酸/氨酸蛋白激M_1 (serine/threonine protein kinase-1, AKT1)、原癌基因(proto-oncogene, JUN 入抑癌基因 TP53 等,主要涉 及营养元素和脂多糖的应答过程、氧化应激反应、类固醇代谢应答过程、脂多糖的反应、活性氧代谢过程、小分子代谢过程的 调控等生物过程。主要富集在磷脂酰肌醇-4, 5-二磷酸3-激酶-蛋白激酶B (phosphatidylinositol-4, 5-biphosphate 3-kinase-pro-tein kinase B, PI3K~Akt)、流体剪应力与动脉粥样硬化、糖尿病并发症中的糖基化终末产物及其(advanced glycation and products-receptor of AGE, AGE-RAGE入丝裂原活化蛋白激酶(mitogerractivated protein kinase, M APK)、TNFp、白介素 17 (interleukin-17, IL-17)等信号通路。结於:益气活血方抗肝纤维化具有多成分、多靶点、多通路的作用特点, 本研究为益气活 血方抗肝纤维化机制的后续实验研究提供了理论基础和参考依据. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
