التفاصيل البيبلوغرافية
| العنوان: |
NADPH–cytochrome P450 reductase knockdown decreases the response to precocene I in the migratory locust Locusta migratoria. |
| المؤلفون: |
Zhang, Yichao1 (AUTHOR) zyc1991@sxu.edu.cn, Li, Hongli1,2 (AUTHOR), Yuan, Shaohang1,2 (AUTHOR), Wu, Haihua1 (AUTHOR), Liu, Xiaojian1 (AUTHOR), Zhang, Jianzhen1 (AUTHOR) |
| المصدر: |
Pesticide Biochemistry & Physiology. Feb2023, Vol. 190, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*MIGRATORY locust, *NICOTINAMIDE adenine dinucleotide phosphate, *FLAVIN adenine dinucleotide, *RECOMBINANT proteins, *FLAVIN mononucleotide, *JUVENILE hormones |
| مستخلص: |
Precocene I is a juvenile hormone antagonist that needs to be activated via oxidative biotransformation catalyzed by cytochrome P450 (CYP). NADPH–cytochrome P450 reductase (CPR) supplies CYP with electrons in the oxidation–reduction process; however, its functional role in the activation of precocene I remains unexplored. Here, the representative characteristics of CPRs were analyzed in the CPR gene of Locusta migratoria (LmCPR), the result of model docking indicated that the hydrogen bonds were formed between reduced nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and NADPH-, FAD-, FMN-domains of LmCPR, respectively. Treating the fourth-instar nymphs with precocene I decreased the juvenile hormone titers of nymphs to 0.55-fold of that in acetone-treated controls, and extended the interval time between fourth- and fifth-instar nymphs. 68.75% of the treated fourth-instar nymphs developed into precocious adults in the fifth-instar. LmCPR knockdown decreased the response to precocene I in the nymphs, the occurrence rate of precocious adults induced by precocene I treatment reduced by 23.11%. Therefore, LmCPR may be involved in the activation of precocene I in L. migratoria. In addition, we generated an active recombinant LmCPR protein using a prokaryotic expression system, its activity in reducing cytochrome c was 33.13 ± 11.50 nmol CytCred/min/μg protein. This study lays the foundation for further research on the role of LmCPR in precocene I activation. Figure. Model illustrating the involvement of LmCPR in the activation of prececone I. [Display omitted] • Precocene I treatment induced the occurrence of precocious adults. • LmCPR knockdown decreased the response to precocene I. • A recombinant LmCPR protein was successfully obtained by prokaryotic expression. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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