دورية أكاديمية
Generation of Multiple Arbovirus-like Particles Using a Rapid Recombinant Vaccinia Virus Expression Platform
| العنوان: | Generation of Multiple Arbovirus-like Particles Using a Rapid Recombinant Vaccinia Virus Expression Platform |
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| المؤلفون: | Yuxiang Wang, Anthony Griffiths, Douglas E. Brackney, Paulo H. Verardi |
| المصدر: | Pathogens, Vol 11, Iss 12, p 1505 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | recombinant vaccinia virus vectors, arboviruses, virus-like particles, Powassan virus, Heartland virus, severe fever with thrombocytopenia syndrome virus, Medicine |
| الوصف: | As demonstrated by the 2015 Zika virus outbreak in the Americas, emerging and re-emerging arboviruses are public health threats that warrant research investment for the development of effective prophylactics and therapeutics. Many arboviral diseases are underreported, neglected, or of low prevalence, yet they all have the potential to cause outbreaks of local and international concern. Here, we show the production of virus-like particles (VLPs) using a rapid and efficient recombinant vaccinia virus (VACV) expression system for five tick- and mosquito-borne arboviruses: Powassan virus (POWV), Heartland virus (HRTV), severe fever with thrombocytopenia syndrome virus (SFTSV), Bourbon virus (BRBV) and Mayaro virus (MAYV). We detected the expression of arbovirus genes of interest by Western blot and observed the expression of VLPs that resemble native virions under transmission electron microscopy. We were also able to improve the secretion of POWV VLPs by modifying the signal sequence within the capsid gene. This study describes the use of a rapid VACV platform for the production and purification of arbovirus VLPs that can be used as subunit or vectored vaccines, and provides insights into the selection of arbovirus genes for VLP formation and genetic modifications to improve VLP secretion and yield. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 11121505 2076-0817 |
| العلاقة: | https://www.mdpi.com/2076-0817/11/12/1505Test; https://doaj.org/toc/2076-0817Test |
| DOI: | 10.3390/pathogens11121505 |
| الوصول الحر: | https://doaj.org/article/1ec8a532368b47bf844d1d30203231b2Test |
| رقم الانضمام: | edsdoj.1ec8a532368b47bf844d1d30203231b2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11121505 20760817 |
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| DOI: | 10.3390/pathogens11121505 |