Effect of Non-Concentrated and Concentrated Vaporized Hydrogen Peroxide on Scrapie Prions
| العنوان: | Effect of Non-Concentrated and Concentrated Vaporized Hydrogen Peroxide on Scrapie Prions |
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| المؤلفون: | Chihiro Harata, Risa Yamashiro, Akikazu Sakudo |
| المصدر: | Pathogens Volume 9 Issue 11 |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), hydrogen peroxide gas, Half cycle, animal diseases, Scrapie, 030501 epidemiology, Article, prion, 03 medical and health sciences, chemistry.chemical_compound, VHP, Mouse bioassay, Immunology and Allergy, inactivation, Hydrogen peroxide, Molecular Biology, 030304 developmental biology, 0303 health sciences, General Immunology and Microbiology, medical device, Sterilization (microbiology), Molecular biology, nervous system diseases, Infectious Diseases, chemistry, Cover glass, Protein Misfolding Cyclic Amplification, Vaporized hydrogen peroxide, 0305 other medical science |
| الوصف: | To date, there have been no studies on the sterilization of prions by non-concentrated and concentrated vaporized hydrogen peroxide (VHP) applied by the same instrument. Here, the effect of the two types of VHP applied using an ES-700 sterilizer on prions was investigated. Brain homogenate from scrapie (Chandler) prion-infected mice was spotted on a cover glass and subjected to ES-700 treatment in soft (non-concentrated VHP from 59% hydrogen peroxide) or standard (concentrated VHP from 80% hydrogen peroxide) mode. Proteinase K-resistant prion protein (PrPres), an indicator of the abnormal isoform of prion protein (PrPSc), was reduced by ES-700 treatment under several conditions: SFT1/4 (soft mode, quarter cycle), SFT1/2 (soft mode, half cycle), SFT1 (soft mode, full cycle), and STD1/2 (standard mode, half cycle). PrPres was detected after the first and second rounds of protein misfolding cyclic amplification (PMCA) of untreated samples, but was undetectable in SFT1/4, SFT1/2, SFT1, and STD1/2 treated samples. In a mouse bioassay, SFT1/2 and STD1/2 treatment of prions significantly prolonged survival time, suggesting that prion infectivity is reduced after ES-700 treatment. In summary, both non-concentrated and concentrated VHP inactivate prions and may be useful for the low-temperature sterilization of prion-contaminated medical devices. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-0817 |
| DOI: | 10.3390/pathogens9110947 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cd697f0a4fbcc9e42c7b65b941cb2a1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2cd697f0a4fbcc9e42c7b65b941cb2a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20760817 |
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| DOI: | 10.3390/pathogens9110947 |