Kupffer Cells Survive Plasmodium berghei Sporozoite Exposure and Respond with a Rapid Cytokine Release
| العنوان: | Kupffer Cells Survive Plasmodium berghei Sporozoite Exposure and Respond with a Rapid Cytokine Release |
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| المؤلفون: | Zhaoli Sun, Rhoel R. Dinglasan, Rebecca E. Tweedell, Le Qi |
| المصدر: | Pathogens Volume 7 Issue 4 Pathogens, Vol 7, Iss 4, p 91 (2018) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Programmed cell death, medicine.medical_treatment, Cell, malaria, lcsh:Medicine, Kupffer cell, 03 medical and health sciences, chemistry.chemical_compound, Plasmodium berghei, Lactate dehydrogenase, parasitic diseases, medicine, Immunology and Allergy, sporozoite, Molecular Biology, innate immunity, Innate immune system, General Immunology and Microbiology, Plasmodium (life cycle), biology, lcsh:R, biology.organism_classification, eye diseases, cytokines, 3. Good health, Cell biology, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Cytokine, cell death, chemistry |
| الوصف: | The liver stage of the Plasmodium life cycle features sporozoite traversal of the liver sinusoidal barrier through Kupffer cells (KCs) followed by invasion of hepatocytes. Little is known about the interaction of Plasmodium sporozoites with KCs, the liver-resident macrophages. Previous reports suggest KCs do not mount a pro-inflammatory response and undergo cell death following this interaction. Our work explores this interaction using primary rat KCs (PRKCs) and Plasmodium berghei sporozoites. We analyzed PRKC culture supernatants for markers of an immunological response through cytokine arrays. Additionally, cell wounding and death were assessed by monitoring lactate dehydrogenase (LDH) levels in these supernatants and by live/dead cell imaging. We found that PRKCs mount an immunological response to P. berghei sporozoites by releasing a diverse set of both pro- and anti-inflammatory cytokines, including IFN&gamma IL-12p70, Mip-3&alpha IL-2, RANTES, IL-1&alpha IL-4, IL-5, IL-13, EPO, VEGF, IL-7, and IL-17&alpha We also observed no difference in LDH level or live/dead staining upon sporozoite exposure, suggesting that the KCs are not deeply wounded or dying. Overall, our data suggest that sporozoites may be actively modulating the KC&rsquo s reaction to their presence and altering the way the innate immune system is triggered by KCs. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-0817 |
| DOI: | 10.3390/pathogens7040091 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8778507cfa82cfa8cfc6260a4c93552Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a8778507cfa82cfa8cfc6260a4c93552 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20760817 |
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| DOI: | 10.3390/pathogens7040091 |