Epidemiology and genetic diversity of Anaplasma ovis in goats in Corsica, France
| العنوان: | Epidemiology and genetic diversity of Anaplasma ovis in goats in Corsica, France |
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| المؤلفون: | Cabezas Cruz, Alejandro, Gallois, Mélanie, Fontugne, Mélanie, Allain, Eléonore, Denoual, Myriam, Moutailler, Sara, Devillers, Elodie, Zientara, Stephan, Memmi, Marc, Chauvin, Alain, Agoulon, Albert, Vayssier Taussat, Muriel, Chartier, Christophe |
| المساهمون: | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Fédération Régionale des Groupements de Défense Sanitaire du Bétail de Corse, Partenaires INRAE, Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de la Recherche Agronomique (INRA), Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Laboratoire d'Analyses de Corse, Département Santé Animale (DEPT SA), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) |
| المصدر: | Parasites & Vectors Parasites and Vectors Parasites and Vectors, BioMed Central, 2019, 12 (1), pp.3. ⟨10.1186/s13071-018-3269-7⟩ Parasites and Vectors 1 (12), 3. (2019) Parasites & Vectors, Vol 12, Iss 1, Pp 1-11 (2019) Parasites and Vectors, 2019, 12 (1), pp.3. ⟨10.1186/s13071-018-3269-7⟩ |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Anaplasmosis, Goat Diseases, [SDV]Life Sciences [q-bio], Goats, Research, Anaplasma ovis, Genetic Variation, Corsica, Sequence Analysis, DNA, Dairy goat, Prevalence, Rhipicephalus bursa, France, Genetic diversity, lcsh:Infectious and parasitic diseases, Dairying, Random Allocation, Rhipicephalus, Animals, lcsh:RC109-216, Female, Sequence Alignment, Phylogeny |
| الوصف: | Background Anaplasma ovis is a major cause of small ruminant anaplasmosis, a tick-borne disease mainly affecting small ruminants in tropical and subtropical regions of the world. Due to health and production problems in dairy goat flocks in Corsica, France, and the demonstration of A. ovis infection in some animals, an extensive survey was conducted in the island in spring 2016. The aim of the survey was to determine the prevalence and geographical distribution of A. ovis infections in goats and ticks as well as possible relationships with anaemia and other health indicators. In addition, the genetic diversity of A. ovis was evaluated. Methods Blood and faecal samples were collected in 55 clinically healthy flocks (10 goats per flock) for A. ovis qPCR, haematocrit determination, paratuberculosis ELISA seropositivity and gastrointestinal nematode egg excretion quantification. Ticks were collected, identified and processed for A. ovis DNA detection. Results A high prevalence of A. ovis DNA detection was found at the individual (52.0%) and flock levels (83.6%) with a within-flock prevalence ranging between 0–100%. Rhipicephalus bursa was the only tick species collected on goats (n = 355) and the detection rate of A. ovis DNA in ticks was 20.3%. Anaplasma ovis DNA prevalence was higher in flocks located at an altitude above 168 m, in goats of Corsican/crossbred breed and in goats > 3 years-old. No relationship was found between A. ovis DNA detection at the individual or flock level and haematocrit, paratuberculosis seropositivity or gastrointestinal parasites. Positive A. ovis goat samples were used for amplification of gltA and msp4 genes for species confirmation and strain identification, respectively. Sequence and phylogenetic analysis of these genes confirmed the detection of A. ovis and allowed identification of six different strains of this pathogen (named Corsica 1-6 (COR1-6). While the msp4 sequence of strain COR1 had 100% identity with strains previously reported, COR2 to 6 were found to be novel strains. The strain COR1 was the most represented, corresponding to 94.6% of the msp4 sequences obtained. Conclusions The results showed a relatively high genetic diversity of A. ovis associated with high bacterial prevalence in goats. Electronic supplementary material The online version of this article (10.1186/s13071-018-3269-7) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| تدمد: | 1756-3305 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8c5cc3f8105b6241651cfb84b6230398Test https://pubmed.ncbi.nlm.nih.gov/30606253Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....8c5cc3f8105b6241651cfb84b6230398 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17563305 |
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