مورد إلكتروني
The kinetics of cellular and humoral immune responses of common carp to presporogonic development of the myxozoan Sphaerospora molnari
| العنوان: | The kinetics of cellular and humoral immune responses of common carp to presporogonic development of the myxozoan Sphaerospora molnari |
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| المصدر: | ISSN: 1756-3305 |
| بيانات النشر: | 2019 |
| تفاصيل مُضافة: | Korytář, Tomáš Wiegertjes, Geert F. Zusková, Eliška Tomanová, Anna Lisnerová, Martina Patra, Sneha Sieranski, Viktor Šíma, Radek Born-Torrijos, Ana Wentzel, Annelieke S. Blasco-Monleon, Sandra Yanes-Roca, Carlos Policar, Tomáš Holzer, Astrid S. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | BACKGROUND: Sphaerospora molnari is a myxozoan parasite causing skin and gill sphaerosporosis in common carp (Cyprinus carpio) in central Europe. For most myxozoans, little is known about the early development and the expansion of the infection in the fish host, prior to spore formation. A major reason for this lack of information is the absence of laboratory model organisms, whose life-cycle stages are available throughout the year. RESULTS: We have established a laboratory infection model for early proliferative stages of myxozoans, based on separation and intraperitoneal injection of motile and dividing S. molnari stages isolated from the blood of carp. In the present study we characterize the kinetics of the presporogonic development of S. molnari, while analyzing cellular host responses, cytokine and systemic immunoglobulin expression, over a 63-day period. Our study shows activation of innate immune responses followed by B cell-mediated immune responses. We observed rapid parasite efflux from the peritoneal cavity (< 40 hours), an initial covert infection period with a moderate proinflammatory response for about 1-2 weeks, followed by a period of parasite multiplication in the blood which peaked at 28 days post-infection (dpi) and was associated with a massive lymphocyte response. Our data further revealed a switch to a massive anti-inflammatory response (up to 1456-fold expression of il-10), a strong increase in the expression of IgM transcripts and increased number of IgM+ B lymphocytes, which produce specific antibodies for the elimination of most of the parasites from the fish at 35 dpi. However, despite the presence of these antibodies, S. molnari invades the liver 42 dpi, where an increase in parasite cell number and indistinguishable outer cell membranes are indicative of effective exploitation and disguise mechanisms. From 49 dpi onwards, the acute infection changes to a chronic one, with low parasite numbers remaining in the fish. CONCLUSIONS: To o |
| مصطلحات الفهرس: | B cells, Cyprinus carpio, Cytokines, Host–parasite interaction, IgM, Myxozoa, Sphaerospora molnari, Teleost, Article/Letter to editor |
| URL: | |
| الإتاحة: | Open access content. Open access content Wageningen University & Research |
| ملاحظة: | application/pdf Parasites & Vectors 12 (2019) 1 ISSN: 1756-3305 ISSN: 1756-3305 English |
| أرقام أخرى: | NLWUP oai:library.wur.nl:wurpubs/550989 https://library.wur.nl/WebQuery/wurpubs/550989Test 10.1186/s13071-019-3462-3 1200319897 |
| المصدر المساهم: | WUR STAFF PUBNS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1200319897 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |