LncRNA TUG1 is upregulated and promotes cell proliferation in osteosarcoma
| العنوان: | LncRNA TUG1 is upregulated and promotes cell proliferation in osteosarcoma |
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| المؤلفون: | Zhang Pei, Feng Yunbo, Liu Xiaopo, Tian Fa-Ming, Li Xiaoli, Cao Guolong |
| المصدر: | Open Medicine, Vol 11, Iss 1, Pp 163-167 (2016) Open Medicine |
| بيانات النشر: | De Gruyter, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene knockdown, Small interfering RNA, Cell growth, business.industry, akt, proliferation, General Medicine, Transfection, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Downregulation and upregulation, Cell culture, 030220 oncology & carcinogenesis, osteosarcoma, Cancer research, Medicine, business, tug1, Protein kinase B, PI3K/AKT/mTOR pathway, Research Article |
| الوصف: | Objective: To examine the expression and function of long non-coding RNA taurine up-regulated 1 (TUG1) in human osteosarcoma cells. Methods: Real-time quantitive PCR was used to detect the transcription level of TUG1 in a series of osteosarcoma cell lines. Knockdown of TUG1 in U2OS cells was carried out by transient transfection of siRNAs. MTT assay was performed to access the cell growth rates. Afterwards, RNA and protein of these cells were extracted to analyze the transfection efficient as well as the expression of other molecules. Results: Compared to the normal cell line, TUG1 exhibited a significant upregulation in osteosarcoma cells. Phenotyping analysis showed the growth-promotion activity of TUG1, since knockdown of TUG1 resulted in declined proliferation. We also found that AKT phosphorylation was impaired after TUG1 was inhibited, suggesting that the AKT pathway was involved in the regulation of TUG1 in U2OS cells. Conclusion: Our data provided evidence that TUG1 was upregulated and acted as a possible oncogene via positively regulating cell proliferation in osteosarcoma cells. |
| اللغة: | English |
| تدمد: | 2391-5463 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2c690182cb01da499acc778dd48f7d5Test https://doaj.org/article/0a9a2e7133e3406398532129d6a62ff5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a2c690182cb01da499acc778dd48f7d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23915463 |
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