CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells
| العنوان: | CCL22 and IL-37 inhibit the proliferation and epithelial-mesenchymal transition process of NSCLC A549 cells |
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| المؤلفون: | Yu-Hua Chen, Si-Si Liang, Yong-Hua Chen, Xian-Jing Wu, Jun-Fa Xu, Bi-Yun Zhou, Jun Ai Zhang |
| المصدر: | Oncology Reports. 36:2017-2024 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Epithelial-Mesenchymal Transition, Lung Neoplasms, Blotting, Western, Cell, Vimentin, Biology, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, 0302 clinical medicine, Plasmid, Carcinoma, Non-Small-Cell Lung, medicine, Humans, MTT assay, Epithelial–mesenchymal transition, Cell Proliferation, Chemokine CCL22, A549 cell, Microscopy, Confocal, Cell growth, General Medicine, Cell cycle, Molecular biology, 030104 developmental biology, medicine.anatomical_structure, Oncology, A549 Cells, 030220 oncology & carcinogenesis, biology.protein, Interleukin-1 |
| الوصف: | In the present study, we aimed to investigate the effects of CC chemokine ligand 22 (CCL22) and interleukin-37 (IL-37) on the proliferation and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) A549 cells. pDsRed-CCL22 and pEGFP-IL-37 plasmids were constructed. A549 cells were divided into six groups: the control, the pDsRed-N1 blank plasmid, the pEGFP-C1 blank plasmid, the pDsRed-CCL22 plasmid, the pEGFP‑IL-37 plasmid and the pDsRed-CCL22 + pEGFP-IL-37 plasmid group. Expression levels and localization of CCL22 and IL-37 in cells were detected by confocal microscopy. Phase-contrast microscopy was applied for observing cellular morphology. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used for detecting the mRNA levels of vimentin, N-cadherin and E-cadherin, and their protein expression levels were tested using western blotting. Constructed plasmids expressed CCL22 and IL-37, both of which had a co-localization in the cell membrane. MTT assay and cell observation results revealed that CCL22 and IL-37 inhibited the proliferation and EMT process of the A549 cells. The results of RT-qPCR and western blotting revealed that decreased vimentin and N-cadherin mRNA and protein expression levels, and increased E-cadherin mRNA and protein expression levels were found in the pDsRed-CCL22 plasmid, pEGFP-IL-37 plasmid and pDsRed‑CCL22 + pEGFP‑IL-37 plasmid groups when compared with the control, the pDsRed-N1 blank plasmid and the pEGFP-C1 blank plasmid groups (all P |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::343ae578c6ab20fca661fbe8f1c236fdTest https://doi.org/10.3892/or.2016.4995Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....343ae578c6ab20fca661fbe8f1c236fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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