Novel and simple method of double‑detection using fluorescence in�situ hybridization and fluorescence immunostaining of formalin‑fixed paraffin‑embedded tissue sections
| العنوان: | Novel and simple method of double‑detection using fluorescence in�situ hybridization and fluorescence immunostaining of formalin‑fixed paraffin‑embedded tissue sections |
|---|---|
| المؤلفون: | Satoshi Ikeda |
| المصدر: | Oncology Letters. |
| بيانات النشر: | Spandidos Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Formalin fixed paraffin embedded, medicine.diagnostic_test, Routine laboratory, Biology, Fluorescence, Molecular biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Tissue sections, Oncology, 030220 oncology & carcinogenesis, Fluorescence microscope, medicine, Immunostaining, Protein overexpression, Fluorescence in situ hybridization, Biomedical engineering |
| الوصف: | The importance of fluorescence in situ hybridization (FISH) for pathological diagnosis has been increasing. However, the procedures utilized for a conventional FISH method with formalin-fixed paraffin-embedded tissue sections are complicated and it is difficult to perform as a routine laboratory test. In addition, there are difficulties with differentiation of targeted cells in observations with a fluorescence microscope. The present study reported a novel method that utilizes FISH in combination with fluorescence immunostaining as a simple double-detection technique that addresses these problems. Using this novel method, various genetic aberrations, as well as protein overexpression were easily visualized in isologous sections. In particular, FISH signals with our method clearly identify target cells in samples with poor differentiation between tumor cells coexisting with normal cells. It is proposed that this simple technique is widely applicable as a routine laboratory test and future developments are expected. |
| تدمد: | 1792-1082 1792-1074 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::fad130dc9cd4aa15c94fdf02b401bb4cTest https://doi.org/10.3892/ol.2017.7413Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........fad130dc9cd4aa15c94fdf02b401bb4c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
|---|