المؤلفون: Fulin Qiang, Qichang Yang, Song He, Qiuhong Wang, Hua Wang, Lei Yang, Jin Cai, Yingying Wang, Jinzhang Xiao

المصدر: Oncology Letters

مصطلحات موضوعية: 0301 basic medicine, Cancer Research, Oncogene, business.industry, gastric cancer, proliferation, Cancer, Articles, Cell cycle, Src-associated in mitosis of 68 kDa, medicine.disease, Malignancy, medicine.disease_cause, migration, Molecular medicine, Metastasis, 03 medical and health sciences, 030104 developmental biology, Oncology, Cancer cell, medicine, Cancer research, metastasis, business, Carcinogenesis

الوصف: Gastric cancer is one of the most common types of malignancy worldwide. However, the molecular mechanisms of cancer development remain unclear. Src-associated in mitosis of 68 kDa (Sam68) is involved in cell proliferation, transformation, tumorigenesis and metastasis in several types of cancer. The present study aimed to assess the expression and function of Sam68 in human gastric cancer. Western blot analysis and immunohistochemistry indicated that Sam68 expression was increased in tumor samples and the levels were associated with the grade of malignancy. High Sam68 expression was associated with the poor prognosis of patients with gastric cancer. In vitro, following knockdown of Sam68 by transfection of gastric cancer cells with small interfering RNA, the cell viability, cell cycle progress, migration and invasion were decreased. The results of the present study revealed that Sam68 may be a novel prognostic factor for, and is associated with cell growth, migration and invasion in, gastric cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8335247b4231a4b806c18902f6a2853aTest
http://europepmc.org/articles/PMC5840748Test

المؤلفون: Ailan Xian, Chunbin Wang, Ni Guo, Zaixing Wang, Longjun Yang, Lei Yang, Jie Gao

المصدر: Oncology Letters

مصطلحات موضوعية: Cancer Research, Respiratory rate, medicine.medical_treatment, Remifentanil, fentanyl, Fentanyl, 03 medical and health sciences, 0302 clinical medicine, 030202 anesthesiology, Heart rate, Medicine, Intubation, digestive endoscopy, propofol, business.industry, Articles, Blood pressure, colon cancer, Oncology, Intravenous anesthesia, Anesthesia, 030211 gastroenterology & hepatology, business, Propofol, remifentanil, medicine.drug

الوصف: Anesthetic effect of remifentanil combined with propofol in awakening painless endoscopy was analyzed. Retrospective analysis of 120 cases of colon cancer were treated in Dongying People's Hospital from June 2015 to December 2017. All of them were treated by awakening painless digestive endoscopy, divided into 60 cases in observation group (combined with remifentanil and propofol anesthesia), and 60 cases in control group (combined intravenous anesthesia of finanib and propofol). The data were respectively recorded at time-points of oxygen inhalation, intubation for 10 min, awakening time, waking time, and the time-points for each represented as the time-points of T1, T2, T3, T4, T5 and recorded the diastolic blood pressure (DBP), respiratory rate (RR) and heart rate (HR), and compared the awakening effect and the occurrence of adverse reaction. There was no significant difference in the DBP index between the two groups at time-point T1 (P>0.05). The time-points of T2, T3, T4 and T5 were significantly different from the observation group (P0.05). Compared with the control group, the awakening time and consciousness recovering of the observation group is lower (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1de87686d614df0a4c55f9a14f6527eTest
https://doi.org/10.3892/ol.2018.9801Test

المؤلفون: Xueyang Yang, Kunyan Liu, Luyong Zhang, Gang Hu, He Zhou, Weikang Tao, Wang Zongyu, Fubo Xie, Kedong Ouyang, Lei Yang, Xuzhen Tang, Yixin Zhang, Jibin Liu, Fang Houshun, Zhenzhou Jiang

المصدر: Oncology Letters

مصطلحات موضوعية: Sorafenib, Cancer Research, patient-derived xenograft, Angiogenesis, Cell, Drug resistance, 03 medical and health sciences, 0302 clinical medicine, In vivo, fibroblast growth factor, Medicine, Protein kinase B, business.industry, acquired resistance, Articles, hepatocellular carcinoma, Cell cycle, digestive system diseases, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, sorafenib, 030211 gastroenterology & hepatology, Human umbilical vein endothelial cell, business, medicine.drug

الوصف: Acquired resistance to targeted therapies is an important clinical challenge. Research focusing on acquired resistance is hindered by the lack of relevant model systems. In the present study, the generation and characterization of an in vivo acquired sorafenib-resistant hepatocellular carcinoma (HCC) xenograft model derived from a patient tumor is reported. A cancer cell line (LIXC-004SR) was generated from a tumor that had developed following ~100 days of sorafenib treatment of a HCC patient-derived xenograft (PDX) model (LIX004). The xenograft tumors derived from this cell line demonstrated sorafenib-resistance in vivo. By contrast, a cell line (LIXC-004NA) generated from a vehicle-treated LIX004 PDX model remained sensitive to sorafenib in vivo. Following treatment with sorafenib in vivo, angiogenesis was significantly elevated in the LIXC-004SR tumors when compared with that in the LIXC-004NA tumors. The LIXC-004SR cell culture supernatant stimulated human umbilical vein endothelial cell proliferation and extracellular-signal-regulated kinase and protein kinase B phosphorylation, which can only be inhibited by the combination of sorafenib and a fibroblast growth factor receptor 1 (FGFR1) inhibitor, AZD4547. The tumor growth of the sorafenib-resistant LIXC-004SR xenograft was inhibited by the FGFR1 inhibitor in vivo, suggesting that one of the underlying mechanisms of the acquired resistance is likely due to activation of alternative angiogenic pathways. The LIXC-004SR cell line also exhibited signs of multi-drug resistance and genetic instability. Taken together, these data suggest that this in vivo model of acquired resistance from a PDX model may reflect sorafenib-resistance in certain patients and may facilitate drug resistance research, as well as contributing to the clinical prevention and management of drug resistance.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13395f3a375e925a2d1d06dbe8265d83Test
https://doi.org/10.3892/ol.2018.9078Test

المؤلفون: Liang Dong, Lei Yang, Jianfeng Xu

المصدر: Oncology Letters.

مصطلحات موضوعية: 0301 basic medicine, Cancer Research, medicine.medical_specialty, Lung, Oncogene, medicine.diagnostic_test, business.industry, H&E stain, Articles, medicine.disease, Molecular medicine, Proinflammatory cytokine, Sepsis, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Endocrinology, medicine.anatomical_structure, Oncology, Western blot, Apoptosis, 030220 oncology & carcinogenesis, Internal medicine, medicine, business

الوصف: Aquaporin 5 (AQP-5) is a water channel protein that is closely associated with non-small cell lung cancer tissues. The present study aimed to investigate the mechanism of tanshinol treatment on AQP-5 in the lung tissue of rats with sepsis. Animals in a rat sepsis model were randomly divided into six groups including blank control (ctrl), sham operation (SO), model (sepsis), low dose tanshinol (5 mg/kg/day; Tan-L), moderate dose tanshinol (10 mg/kg/day; Tan-M) and high dose tanshinol (20 mg/kg/day; Tan-H) groups. After 7 days of administration, the expression level of AQP-5 mRNA was detected by reverse transcription-quantitative polymerase chain reaction. The levels of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) were measured by ELISA. Hematoxylin and eosin staining was used for histopathological observation. The expression levels of AQP-5, P38 and phosphorylated (P)-P38 protein in lung tissues were detected by western blot analysis. The expression levels of AQP-5 in the sepsis group were significantly decreased compared with those in ctrl and SO groups (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01b8d87955c25075bb9c8f1d3b1114baTest
https://doi.org/10.3892/ol.2018.9026Test

المؤلفون: Jun‑Cheng Wang, Ming‑Lei Yang, Ding‑Kang Yao, Wen Bin Zou

المصدر: Oncology letters. 16(4)

مصطلحات موضوعية: Cancer Research, Univariate analysis, medicine.medical_specialty, Gastrointestinal bleeding, Abdominal pain, GiST, business.industry, Stomach, Hazard ratio, Cancer, medicine.disease, Gastroenterology, Metastasis, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Internal medicine, Medicine, 030211 gastroenterology & hepatology, medicine.symptom, business

الوصف: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms in the gastrointestinal tract, exhibiting wide variability in their biological behavior. The aim of the present study was to investigate the clinicopathological characteristics and prognostic factors of GISTs in Chinese patients. All GIST cases (n=182) retrieved from the pathology database and the archived files in Shanghai Changzheng Hospital between January 2011 and December 2014 were reviewed. The clinical symptoms, preoperative investigations, treatments, pathological characteristics and follow-up data of these patients were reviewed, and univariate and multivariate survival analyses were performed. A total of 73.1% of the GISTs were located in the stomach, and the most common three symptoms included abdominal pain (30.2%), dyspepsia (23.1%) and gastrointestinal bleeding (21.4%). Univariate analysis revealed that larger tumor size (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d4ff08f8f986f4dbc770efcdd0b81a9Test
https://pubmed.ncbi.nlm.nih.gov/30250556Test

المؤلفون: Haowen Zhang, Lei Yang, Xiaopeng Yuan, Cheng Gu, Fenju Liu, Jie Wang, Jiahua Yu

المصدر: Oncology Letters. 10:473-478

مصطلحات موضوعية: Cancer Research, business.industry, Kinase, Articles, Cell cycle, medicine.disease, Oncology, Apoptosis, Glioma, Immunology, medicine, Cancer research, Radiosensitivity, Cyclin-dependent kinase 7, business, Protein kinase A, hormones, hormone substitutes, and hormone antagonists, Protein kinase C

الوصف: The present study aimed to investigate the radiosensitizing effects of tamoxifen (TAM), a non-steroidal anti-estrogen drug, in human glioma A172 and U251 cells in vitro. A colony-forming assay revealed that TAM enhances radiosensitivity in A172 and U251 cells. Treatment with TAM also increased the percentage of apoptotic cells subsequent to ionizing radiation, and increased the expression of apoptotic markers, including cleaved caspase-3 and poly(ADP-ribose) polymerase. Ionizing radiation induced G2/M phase arrest, which was alleviated within 24 h when the radiation-induced DNA damage was repaired. However, flow cytometry analysis revealed that TAM treatment delayed the recovery of cell cycle progression. Additional examination demonstrated that TAM-mediated protein kinase C-ι (PKC-ι) inhibition may lead to the activation of pro-apoptotic B-cell lymphoma 2-associated death promoter, and the dephosphorylation of cyclin-dependent kinase 7, resulting in increased cell apoptosis and sustained G2/M phase arrest following exposure to radiation. The present data indicate that the radiosensitizing effects of TAM on glioma cells are partly due to the inhibition of PKC-ι activity in vitro.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::444ca2e6f3a28b3454ce8c11eb09c582Test
https://doi.org/10.3892/ol.2015.3195Test