Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro
| العنوان: | Radiosensitization of human glioma cells by tamoxifen is associated with the inhibition of PKC-ι activity in vitro |
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| المؤلفون: | Haowen Zhang, Lei Yang, Xiaopeng Yuan, Cheng Gu, Fenju Liu, Jie Wang, Jiahua Yu |
| المصدر: | Oncology Letters. 10:473-478 |
| بيانات النشر: | Spandidos Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cancer Research, business.industry, Kinase, Articles, Cell cycle, medicine.disease, Oncology, Apoptosis, Glioma, Immunology, medicine, Cancer research, Radiosensitivity, Cyclin-dependent kinase 7, business, Protein kinase A, hormones, hormone substitutes, and hormone antagonists, Protein kinase C |
| الوصف: | The present study aimed to investigate the radiosensitizing effects of tamoxifen (TAM), a non-steroidal anti-estrogen drug, in human glioma A172 and U251 cells in vitro. A colony-forming assay revealed that TAM enhances radiosensitivity in A172 and U251 cells. Treatment with TAM also increased the percentage of apoptotic cells subsequent to ionizing radiation, and increased the expression of apoptotic markers, including cleaved caspase-3 and poly(ADP-ribose) polymerase. Ionizing radiation induced G2/M phase arrest, which was alleviated within 24 h when the radiation-induced DNA damage was repaired. However, flow cytometry analysis revealed that TAM treatment delayed the recovery of cell cycle progression. Additional examination demonstrated that TAM-mediated protein kinase C-ι (PKC-ι) inhibition may lead to the activation of pro-apoptotic B-cell lymphoma 2-associated death promoter, and the dephosphorylation of cyclin-dependent kinase 7, resulting in increased cell apoptosis and sustained G2/M phase arrest following exposure to radiation. The present data indicate that the radiosensitizing effects of TAM on glioma cells are partly due to the inhibition of PKC-ι activity in vitro. |
| تدمد: | 1792-1082 1792-1074 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::444ca2e6f3a28b3454ce8c11eb09c582Test https://doi.org/10.3892/ol.2015.3195Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....444ca2e6f3a28b3454ce8c11eb09c582 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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