FRA16D common chromosomal fragile site oxido-reductase (FOR/WWOX) protects against the effects of ionizing radiation in Drosophila
| العنوان: | FRA16D common chromosomal fragile site oxido-reductase (FOR/WWOX) protects against the effects of ionizing radiation in Drosophila |
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| المؤلفون: | Alison Perkins, Robert Saint, Robert I. Richards, Louise V. O'Keefe, Sonia Dayan, Yinghong Liu |
| المصدر: | Oncogene. 24:6590-6596 |
| بيانات النشر: | Springer Science and Business Media LLC, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | WWOX, Cancer Research, Embryo, Nonmammalian, Biology, Radiation, Ionizing, Genetics, Animals, Drosophila Proteins, Humans, Cloning, Molecular, Molecular Biology, Gene, Conserved Sequence, Regulation of gene expression, Base Sequence, Chromosome Fragile Sites, Tumor Suppressor Proteins, Chromosomal fragile site, Gene Expression Regulation, WW Domain-Containing Oxidoreductase, Chromosome Fragile Site, Larva, Mutation, Drosophila, Chromosome breakage, Oxidoreductases, Homologous recombination, Drosophila Protein |
| الوصف: | Fragile sites are chromosomal structures that have been proposed to have a determining role in cancer-associated DNA instability. The human WWOX gene spans the FRA16D chromosomal fragile site, the common minimal region of homozygous deletion found in adenocarcinomas and three out of five translocation breakpoints in multiple myeloma. Transcripts from the alternatively spliced WWOX gene encode proteins with common N-terminal WW domains and variable homology to the oxidoreductase family of proteins. In this study, the Drosophila orthologue of the WWOX gene was identified and subjected to mutagenesis via homologous recombination. The resultant DmWWOX1 mutants were viable but exhibited an increased sensitivity to ionizing radiation. This radiation sensitivity was rescued by reintroduction and expression of either the wild-type Drosophila or human WWOX genes. Thus, the protective function of DmWWOX in response to irradiation in Drosophila is conserved with human WWOX (hWWOX). This is consistent with a protective role for hWWOX where aberrant expression, as a result of breakage at the associated fragile site, could contribute directly to cancer progression. |
| تدمد: | 1476-5594 0950-9232 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef29553dea5c47279eab5d8852fc7189Test https://doi.org/10.1038/sj.onc.1208806Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ef29553dea5c47279eab5d8852fc7189 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765594 09509232 |
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