Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3-beta-HSD and ARO but lower 5-alpha-R-1 in male rats
| العنوان: | Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3-beta-HSD and ARO but lower 5-alpha-R-1 in male rats |
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| المؤلفون: | Maria Florencia Andreoli, Jorge G. Ramos, Cora Stoker, Gisela Paola Lazzarino, Enrique H. Luque, María Florencia Rossetti |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Elsevier BV, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Cholestenone 5 alpha-Reductase, Endocrinology, Diabetes and Metabolism, Gene Expression, Estrogen receptor, Eating, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Neurotransmitter Agents, Nutrition and Dietetics, Steroidogenic acute regulatory protein, food and beverages, purl.org/becyt/ford/3.1 [https], Medicina Básica, Hypothalamus, purl.org/becyt/ford/3 [https], Signal Transduction, endocrine system, medicine.medical_specialty, 3-Hydroxysteroid Dehydrogenases, CIENCIAS MÉDICAS Y DE LA SALUD, Neuroactive steroid, Neurociencias, Alpha (ethology), Phytoestrogens, Biology, Diet, High-Fat, 03 medical and health sciences, Aromatase, Internal medicine, medicine, Animals, Obesity, Rats, Wistar, Steroidogenic Enzymes, Appetite Regulation, urogenital system, Cholesterol side-chain cleavage enzyme, Allopregnanolone, Estrogen Receptor alpha, Diet, 030104 developmental biology, chemistry, Energy Intake, 030217 neurology & neurosurgery, Erα |
| الوصف: | Removing dietary phytoestrogens causes obesity and diabetes in adult male rats. Based on the facts that hypothalamic food intake control is disrupted in phytoestrogen-deprived animals and that several steroids affect food intake, we hypothesized that phytoestrogen withdrawal alters the expression of hypothalamic steroidogenic enzymes. Male Wistar rats fed with a high-phytoestrogen diet from conception to adulthood were subjected to phytoestrogen withdrawal by feeding them a low-phytoestrogen diet or a high-phytoestrogen, high-fat diet. Withdrawal of dietary phytoestrogens increased 3β-hydroxysteroid dehydrogenase and P450 aromatase gene expression and decreased those of 5α-reductase-1. This is a direct effect of the lack of dietary phytoestrogens and not a consequence of obesity, as it was not observed in high-fat–fed rats. Phytoestrogen withdrawal and high-fat diet intake reduced hypothalamic expression of estrogen receptor (ER)α correlated with low levels of ERα-O, ERα-OS, and ERα-OT transcripts. Variations in gene expression of steroidogenic enzymes may affect the content of neurosteroids. As neurosteroids are related to food intake control, the changes observed may be a novel mechanism in the regulation of energy balance in obese phytoestrogen-deprived animals. In rats, steroidogenesis and ER signaling appear to be altered by phytoestrogen withdrawal in the rat. The ubiquity of phytoestrogens in the diet and changing intakes or withdrawal suggest that aspects of human health could be affected based on the rat and warrant further research. Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Stoker, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 0271-5317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f441875b62b458e97673ea146d086fd7Test https://doi.org/10.1016/j.nutres.2016.05.003Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f441875b62b458e97673ea146d086fd7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02715317 |
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