التفاصيل البيبلوغرافية
| العنوان: |
A higher degree of LINE-1 methylation in peripheral blood mononuclear cells, a one-carbon nutrient related epigenetic alteration, is associated with a lower risk of developing cervical intraepithelial neoplasia |
| المؤلفون: |
Piyathilake, Chandrika J.1 piyathic@uab.edu, Macaluso, Maurizio2, Alvarez, Ronald D.3, Chen, Min1, Badiga, Suguna1, Siddiqui, Nuzhat R.1, Edberg, Jeffrey C.4, Partridge, Edward E.5, Johanning, Gary L.6 |
| المصدر: |
Nutrition. May2011, Vol. 27 Issue 5, p513-519. 7p. |
| مصطلحات موضوعية: |
*BLOOD cell physiology, *DYSPLASIA, *BIOMARKERS, *CARBON, *CERVICAL cancer, *DIET, *DNA, *GENETICS, *METHYLATION, *NUTRITION, *NUTRITIONAL requirements, *SULFITES, *MICRONUTRIENTS, *PERIMENOPAUSE, *DATA analysis, *BODY mass index, *DISEASE risk factors, *CANCER risk factors |
| مستخلص: |
Abstract: Objective: The objective of the study was to evaluate LINE-1 methylation as an intermediate biomarker for the effect of folate and vitamin B12 on the occurrence of higher grades of cervical intraepithelial neoplasia (CIN ≥2). Methods: This study included 376 women who tested positive for high-risk human papillomaviruses and were diagnosed with CIN ≥2 (cases) or CIN ≤1 (non-cases). CIN ≥2 (yes/no) was the dependent variable in logistic regression models that specified the degree of LINE-1 methylation of peripheral blood mononuclear cells (PBMCs) and of exfoliated cervical cells (CCs) as the independent predictors of primary interest. In analyses restricted to non-cases, PBMC LINE-1 methylation (≥70% versus <70%) and CC LINE-1 methylation (≥54% versus <54%) were the dependent variables in logistic regression models that specified the circulating concentrations of folate and vitamin B12 as the primary independent predictors. Results: Women in the highest tertile of PBMC LINE-1 methylation had 56% lower odds of being diagnosed with CIN ≥2 (odds ratio 0.44, 95% confidence interval 0.24–0.83, P = 0.011), whereas there was no significant association between degree of CC LINE-1 methylation and CIN ≥2 (odds ratio 0.86, 95% confidence interval 0.51–1.46, P = 0.578). Among non-cases, women with supraphysiologic concentrations of folate (>19.8 ng/mL) and sufficient concentrations of plasma vitamin B12 (≥200.6 ng/mL) were significantly more likely to have highly methylated PBMCs compared with women with lower folate and lower vitamin B12 (odds ratio 3.92, 95% confidence interval 1.06–14.52, P = 0.041). None of the variables including folate and vitamin B12 were significantly associated with CC LINE-1 methylation. Conclusion: These results suggest that a higher degree of LINE-1 methylation in PBMCs, a one-carbon nutrient-related epigenetic alteration, is associated with a lower risk of developing CIN. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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