SUMO modification system facilitates the exchange of histone variant H2A.Z-2 at DNA damage sites
| العنوان: | SUMO modification system facilitates the exchange of histone variant H2A.Z-2 at DNA damage sites |
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| المؤلفون: | Masayuki Kusakabe, Hitoshi Kurumizaka, Yoshiaki Kiuchi, Masahiko Harata, Naoki Horikoshi, Masae Ikura, Hiroki Shima, Satoshi Tashiro, Yasunori Horikoshi, Atsuhiko Fukuto, Tsuyoshi Ikura, Jiying Sun, Kazuhiko Igarashi |
| المصدر: | Nucleus |
| بيانات النشر: | Taylor & Francis, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, animal structures, DNA damage, SUMO-1 Protein, SUMO protein, Histone exchange, Histones, 03 medical and health sciences, chemistry.chemical_compound, Histone code, Humans, Original Research, histone variant, biology, Cell Biology, DNA, Chromatin, Ubiquitin ligase, Cell biology, H2A.Z-2, 030104 developmental biology, Histone, chemistry, SUMO, embryonic structures, biology.protein, PIAS4, Protein Processing, Post-Translational, HeLa Cells |
| الوصف: | Histone exchange and histone post-translational modifications play important roles in the regulation of DNA metabolism, by re-organizing the chromatin configuration. We previously demonstrated that the histone variant H2A.Z-2 is rapidly exchanged at damaged sites after DNA double strand break induction in human cells. In yeast, the small ubiquitin-like modifier (SUMO) modification of H2A.Z is involved in the DNA damage response. However, whether the SUMO modification regulates the exchange of human H2A.Z-2 at DNA damage sites remains unclear. Here, we show that H2A.Z-2 is SUMOylated in a damage-dependent manner, and the SUMOylation of H2A.Z-2 is suppressed by the depletion of the SUMO E3 ligase, PIAS4. Moreover, PIAS4 depletion represses the incorporation and eviction of H2A.Z-2 at damaged sites. These findings demonstrate that the PIAS4-mediated SUMOylation regulates the exchange of H2A.Z-2 at DNA damage sites. |
| اللغة: | English |
| تدمد: | 1949-1042 1949-1034 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82ad85d7341d9caba3c9eb75ba12a941Test http://europepmc.org/articles/PMC5973225Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....82ad85d7341d9caba3c9eb75ba12a941 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19491042 19491034 |
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