Condensin aids sister chromatid decatenation by topoisomerase II
| العنوان: | Condensin aids sister chromatid decatenation by topoisomerase II |
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| المؤلفون: | Adrian Charbin, Frank Uhlmann, Céline Bouchoux |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press (OUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Adenosine Triphosphatases, Genetics, biology, Condensin, Cell Cycle, DNA replication, Saccharomyces cerevisiae, macromolecular substances, Genome Integrity, Repair and Replication, Chromatids, DNA, Catenated, Cell biology, DNA-Binding Proteins, Establishment of sister chromatid cohesion, Chromosome segregation, Condensin complex, DNA Topoisomerases, Type II, Chromosome Segregation, Multiprotein Complexes, biology.protein, Sister chromatids, Chromatid, Anaphase |
| الوصف: | The condensin complex is a key determinant of mitotic chromosome architecture. In addition, condensin promotes resolution of sister chromatids during anaphase, a function that is conserved from prokaryotes to human. Anaphase bridges observed in cells lacking condensin are reminiscent of chromosome segregation failure after inactivation of topoisomerase II (topo II), the enzyme that removes catenanes persisting between sister chromatids following DNA replication. Circumstantial evidence has linked condensin to sister chromatid decatenation but, because of the difficulty of observing chromosome catenation, this link has remained indirect. Alternative models for how condensin facilitates chromosome resolution have been put forward. Here, we follow the catenation status of circular minichromosomes of three sizes during the Saccharomyeces cerevisiae cell cycle. Catenanes are produced during DNA replication and are for the most part swiftly resolved during and following S-phase, aided by sister chromatid separation. Complete resolution, however, requires the condensin complex, a dependency that becomes more pronounced with increasing chromosome size. Our results provide evidence that condensin prevents deleterious anaphase bridges during chromosome segregation by promoting sister chromatid decatenation. |
| تدمد: | 1362-4962 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac1257a9b305a2737ed0d68f5fd27cb0Test https://doi.org/10.1093/nar/gkt882Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac1257a9b305a2737ed0d68f5fd27cb0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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