التفاصيل البيبلوغرافية
| العنوان: |
Quantitative assay of element mass inventories in single cell biological systems with micro-PIXE. |
| المؤلفون: |
Ogrinc, Nina1,2, Pelicon, Primož1 primoz.pelicon@ijs.si, Vavpetič, Primož1, Kelemen, Mitja1, Grlj, Nataša1, Jeromel, Luka1, Tomić, Sergej3, Čolić, Miodrag3,4, Beran, Alfred5 |
| المصدر: |
Nuclear Instruments & Methods in Physics Research Section B. Jul2013, Vol. 306, p121-124. 4p. |
| مصطلحات موضوعية: |
*QUANTITATIVE research, *BACKSCATTERING, *SCANNING transmission electron microscopy, *GOLD nanoparticles, *DENDRITIC cells, *ZOOPLANKTON |
| مستخلص: |
Abstract: Elemental concentrations in micro-PIXE (Particle Induced X-ray Emission) maps of elements in biological tissue slices have been determined using auxiliary information on the sample matrix composition from EBS (Elastic Backscattering Spectroscopy) and STIM (Scanning Transmission Ion Microscopy). The thin sample approximation may be used for evaluating micro-PIXE data in cases, where X-ray absorption in the sample can be neglected and the mass of elements in a selected area can be estimated. The resulting sensitivity amounts to an impressive 10−12 g of the selected elements. Two cases are presented as examples. In the first, we determined the total mass of gold nanoparticles internalized by human monocyte-derived dendritic cells (MDDC). In the second, an inventory of the mass of elements in the micro-particulate material adsorbed at the wall of the lorica of the microzooplankton species Tintinnopsis radix has been created. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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