دورية أكاديمية
A combined microphysiological-computational omics approach in dietary protein evaluation
| العنوان: | A combined microphysiological-computational omics approach in dietary protein evaluation |
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| المؤلفون: | Paulus G. M. Jochems, Willem R. Keusters, Antoine H. P. America, Pascale C. S. Rietveld, Shanna Bastiaan-Net, Renata M. C. Ariëns, Monic M. M. Tomassen, Fraser Lewis, Yang Li, Koen G. C. Westphal, Johan Garssen, Harry J. Wichers, Jeroen van Bergenhenegouwen, Rosalinde Masereeuw |
| المصدر: | npj Science of Food, Vol 4, Iss 1, Pp 1-9 (2020) |
| بيانات النشر: | Nature Portfolio, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Food processing and manufacture |
| مصطلحات موضوعية: | Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| الوصف: | Abstract Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to biological parameters. Here, a combined experimental and computational approach is presented, which compared seventeen protein sources with cow’s whey protein concentrate (WPC) as the benchmark. In vitro digestion of proteins was followed by proteomics analysis and statistical model-based clustering. Information on digesta peptide composition resulted in 3 cluster groups, primarily driven by the peptide overlap with the benchmark protein WPC. Functional protein data was then incorporated in the computational model after evaluating the effects of eighteen protein digests on intestinal barrier integrity, viability, brush border enzyme activity, and immune parameters using a bioengineered intestine as microphysiological gut system. This resulted in 6 cluster groups. Biological clustering was driven by viability, brush border enzyme activity, and significant differences in immune parameters. Finally, a combination of proteomic and biological efficacy data resulted in 5 clusters groups, driven by a combination of digesta peptide composition and biological effects. The key finding of our holistic approach is that protein source (animal, plant or alternative derived) is not a driving force behind the delivery of bioactive peptides and their biological efficacy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2396-8370 |
| العلاقة: | https://doaj.org/toc/2396-8370Test |
| DOI: | 10.1038/s41538-020-00082-z |
| الوصول الحر: | https://doaj.org/article/8b3824adfd8543ed9a5b564af4ad6739Test |
| رقم الانضمام: | edsdoj.8b3824adfd8543ed9a5b564af4ad6739 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23968370 |
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| DOI: | 10.1038/s41538-020-00082-z |