Polyunsaturated Fatty Acids Protect Against Prion-Mediated Synapse Damage In Vitro
| العنوان: | Polyunsaturated Fatty Acids Protect Against Prion-Mediated Synapse Damage In Vitro |
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| المؤلفون: | Mourad Tayebi, Alun Williams, Luisa Diomede, Clive Bate, Mario Salmona |
| المصدر: | Neurotoxicity Research. 17:203-214 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Simvastatin, Docosahexaenoic Acids, Prions, animal diseases, Synaptophysin, Enzyme-Linked Immunosorbent Assay, In Vitro Techniques, Hippocampal formation, Neurotransmission, Toxicology, Hippocampus, Synapse, Mice, Animals, Humans, Neurotoxin, Drug Interactions, Cells, Cultured, Cerebral Cortex, Mice, Knockout, Neurons, chemistry.chemical_classification, Dose-Response Relationship, Drug, biology, Caspase 3, General Neuroscience, Embryo, Mammalian, Eicosapentaenoic acid, Peptide Fragments, nervous system diseases, Cell biology, Neuroprotective Agents, Eicosapentaenoic Acid, Gene Expression Regulation, nervous system, chemistry, Biochemistry, Docosahexaenoic acid, Fatty Acids, Unsaturated, biology.protein, lipids (amino acids, peptides, and proteins), Hydroxymethylglutaryl-CoA Reductase Inhibitors, Synaptosomes, Polyunsaturated fatty acid |
| الوصف: | A loss of synapses is characteristic of the early stages of the prion diseases. Here we modelled the synapse damage that occurs in prion diseases by measuring the amount of synaptophysin, a pre-synaptic membrane protein essential for neurotransmission, in cortical or hippocampal neurones incubated with the disease associated isoform of the prion protein (PrP(Sc)), or with the prion-derived peptide PrP82-146. The addition of PrP(Sc) or PrP82-146 caused a dose-dependent reduction in the synaptophysin content of PrP wildtype neurones indicative of synapse damage. They did not affect the synaptophysin content of PrP null neurones. The loss of synaptophysin in PrP wildtype neurones was preceded by the accumulation of PrP82-146 within synapses. Since supplements containing polyunsaturated fatty acids (PUFA) are frequently taken for their perceived health benefits including reported amelioration of neurodegenerative conditions, the effects of some common PUFA on prion-mediated synapse damage were examined. Pre-treatment of cortical or hippocampal neurones with docosahexaenoic (DHA) or eicosapentaenoic acids (EPA) protected neurones against the loss of synaptophysin induced by PrP82-146 or PrP(Sc). This effect of DHA and EPA was selective as they did not alter the loss of synaptophysin induced by a snakevenom neurotoxin. The effects of DHA and EPA were associated with a significant reduction in the amount of FITC-PrP82-146 that accumulated within synapses. Such observations raise the possibility that supplements containing PUFA may protect against the synapse damage and cognitive loss seen during the early stages of prion diseases. |
| تدمد: | 1476-3524 1029-8428 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5351409a315ff0f09ced6daba2de3c03Test https://doi.org/10.1007/s12640-009-9093-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5351409a315ff0f09ced6daba2de3c03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14763524 10298428 |
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