Electrophysiological study of the interactive role of the cannabinoid breakdown inhibitors and L-type calcium channels on granular neurons in the hippocampal dentate gyrus in rats
| العنوان: | Electrophysiological study of the interactive role of the cannabinoid breakdown inhibitors and L-type calcium channels on granular neurons in the hippocampal dentate gyrus in rats |
|---|---|
| المؤلفون: | Alireza Komaki, Masoumeh Kourosh Arami, Siamak Shahidi, Fatemeh Kazemi, Hamidreza Komaki, Seyed Asaad Karimi |
| المصدر: | Neurological Research. 44:446-454 |
| بيانات النشر: | Informa UK Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Calcium Channels, L-Type, Long-Term Potentiation, Pharmacology, Hippocampus, Verapamil Hydrochloride, LTP induction, medicine, Animals, Rats, Wistar, Neurons, Cannabinoids, Chemistry, Dentate gyrus, Population spike, Long-term potentiation, General Medicine, Electric Stimulation, Rats, Verapamil, Neurology, Dentate Gyrus, Synaptic plasticity, Excitatory postsynaptic potential, Neurology (clinical), Endocannabinoids, medicine.drug |
| الوصف: | The interaction between L-type voltage-dependent Ca2+ channels and the endocannabinoid system (eCs) in synaptic plasticity is controversial. In the present research, the impact of acute administration of URB597, as an endocannabinoid breakdown inhibitor, was evaluated after chronic injection of verapamil, as a Ca2+ channels blocker, on inducing long-term potentiation (LTP) in the rat's hippocampal dentate gyrus (DG). Treatment of male Wistar rats was done using intraperitoneal(i.p) injection of verapamil hydrochloride (n = 8) and saline (n = 10), as the solvent of verapamil once a day within 13 days. Anesthetization was done by i.p injection of urethane and the rats were located in the stereotaxic apparatus for surgery, electrode implantation, and field potential recording. After observing a steady-state baseline response, saline or URB597 were injected (n = 9). Measurement of the population spike (PS) amplitude and slope of field excitatory postsynaptic potentials (fEPSPs) in the DG region was performed as a result of perforant pathway (PP) stimulation. Our treatments could inhibit LTP. Our results indicated that the chronic administration of verapamil produced a significant decrease in the slope of fEPSP and PS amplitude. Also, acute URB597 administration decreased the slope of fEPSP and PS amplitude compared to the saline group. Moreover, URB597 administration in combination with chronic administration of verapamil produced a greater decrease in fEPSP slope and PS amplitude than the saline group. These findings indicated that verapamil and URB597 disrupted LTP induction in the DG. Moreover, an interaction was observed between Ca2+ channels and eCs. Therefore, the eCs possibly play a selective role in synaptic plasticity. |
| تدمد: | 1743-1328 0161-6412 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba1e2a234ea4a76766c4f59b0000d2d3Test https://doi.org/10.1080/01616412.2021.2004364Test |
| رقم الانضمام: | edsair.doi.dedup.....ba1e2a234ea4a76766c4f59b0000d2d3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17431328 01616412 |
|---|