التفاصيل البيبلوغرافية
| العنوان: |
NEUROPEPTIDE Y RECEPTORS FROM CALF BRAIN: EFFECT OF CRUDE CONUS VENOM PREPARATIONS ON [ 3 H]NPY BINDING |
| المؤلفون: |
Eva Czerwiec, Georges Vauquelin, Jean-Paul De Backer, Patrick Vanderheyden |
| المصدر: |
Neurochemistry International. 29:669-676 |
| بيانات النشر: |
Elsevier BV, 1996. |
| سنة النشر: |
1996 |
| مصطلحات موضوعية: |
Agonist, medicine.medical_specialty, medicine.drug_class, Mollusk Venoms, Venom, Biology, Tritium, Binding, Competitive, Hippocampus, Radioligand Assay, Cellular and Molecular Neuroscience, Internal medicine, mental disorders, Conus, medicine, Radioligand, Animals, Neuropeptide Y, Receptor, IC50, Cell Biology, Neuropeptide Y receptor, biology.organism_classification, humanities, Frontal Lobe, Receptors, Neuropeptide Y, Logistic Models, Endocrinology, Cattle |
| الوصف: |
NPY receptors are identified in calf frontal cortex and hippocampus membrane preparations by binding of N-[propionyl-3H] neuropeptide Y. Saturation and competition binding data with PYY, NPY-(18–36) and NPY itself fit with a single class of sites: for the radioligand KD = 1.4 ± 0.5 nM, Bmax = 434 ± 180 fmol/mg protein in frontal cortex, KD = 0.7 ± 0.2 nM, Bmax = 267 ± 50 fmol/mg protein in hippocampus. Competition curves of the Y1-subtype selective agonist [Leu31, Pro34]NPY are biphasic in both membrane preparations: high affinity sites (i.e. Y1-subtype) amount to 80% in frontal cortex and 23% in hippocampus. The remaining sites are of the Y2-subtype. Out of 23 Conus venom preparations, 17 inhibit the binding of [3H]NPY in both membrane preparations, but only two of them (from Conus aulicus and C. pennaceus) do so with high potency (ic50 < 5 μg protein/ml). Only one venom preparation (from C. mercator) had weak discriminatory properties (ic50Y2/ic50Y1 = 6). Venom from C. anemone increased the [3H]NPY binding 5-fold and with an ic50 of 15–18 μg protein/ml. This binding occurred to the venom itself and was unrelated to the NPY receptors since it was equally potent when displaced by [Leu31, Pro34]NPY, NPY-(18–36), PYY and NPY. Copyright © 1996 Elsevier Science Ltd |
| تدمد: |
0197-0186 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a20d4b5ab0e0eea6955e971f069a3ba3Test
https://doi.org/10.1016/s0197-0186Test(96)00066-6 |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi.dedup.....a20d4b5ab0e0eea6955e971f069a3ba3 |
| قاعدة البيانات: |
OpenAIRE |
