Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
| العنوان: | Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development |
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| المؤلفون: | Jack Waters, Pavel Osten, Michael Brecht, Winfried Denk, Ali Cetin, Pawel Licznerski, Shoji Komai |
| المساهمون: | Neurosciences |
| المصدر: | Nature Neuroscience Nature Neuroscience, 9(9), 1125-1133. Nature Publishing Group |
| بيانات النشر: | Nature Publishing Group, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Small interfering RNA, Green Fluorescent Proteins, DNA, Recombinant, Sensory system, Biology, Somatosensory system, Synaptic Transmission, Sodium Channels, Postsynaptic potential, medicine, Animals, Sensory deprivation, Sensory cortex, RNA, Small Interfering, Rats, Wistar, Microscopy, Confocal, Neuronal Plasticity, General Neuroscience, Calcium channel, Pyramidal Cells, Lentivirus, Excitatory Postsynaptic Potentials, Somatosensory Cortex, Barrel cortex, Rats, medicine.anatomical_structure, RNA Interference, Sensory Deprivation, Neuroscience |
| الوصف: | Sensory experience is necessary for normal cortical development. This has been shown by sensory deprivation and pharmacological perturbation of the cortex. Because these manipulations affect the cortical network as a whole, the role of postsynaptic cellular properties during experience-dependent development is unclear. Here we addressed the developmental role of somatodendritic excitability, which enables postsynaptic spike timing-dependent forms of plasticity, in rat somatosensory cortex. We used short interfering RNA (siRNA)-based knockdown of Na+ channels to suppress the somatodendritic excitability of small numbers of layer 2/3 pyramidal neurons in the barrel cortex, without altering the ascending sensory pathway. In vivo recordings from siRNA-expressing cells revealed that this manipulation interfered with the normal developmental strengthening of sensory responses. The sensory responsiveness of neighboring cortical neurons was unchanged, indicating that the cortical network was unchanged. We conclude that somatodendritic excitability of the postsynaptic neuron is needed for the regulation of synaptic strength in the developing sensory cortex. |
| وصف الملف: | application/pdf |
| تدمد: | 1546-1726 1097-6256 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30bf648c1a711af59f53409414ccfbaeTest https://pure.eur.nl/en/publications/d915a146-f517-4c4e-a358-5758933fa18fTest |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....30bf648c1a711af59f53409414ccfbae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15461726 10976256 |
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