Genome-wide distribution of linker histone H1.0 is independent of MeCP2
| العنوان: | Genome-wide distribution of linker histone H1.0 is independent of MeCP2 |
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| المؤلفون: | Laura A. Lavery, Steven Andrew Baker, Huda Y. Zoghbi, Yingyao Shao, Aya Ito-Ishida, Hari Krishna Yamalanchili, Ji-Yoen Kim, Laura Dean Heckman, Zhandong Liu, Laura Marie Lombardi, Yaling Sun |
| المصدر: | Nature neuroscience |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Chromatin Immunoprecipitation, congenital, hereditary, and neonatal diseases and abnormalities, endocrine system diseases, Methyl-CpG-Binding Protein 2, Polymerase Chain Reaction, Genome, Article, MECP2, Histones, Mice, 03 medical and health sciences, Prosencephalon, Histone H1, In vivo, mental disorders, Animals, Cell Nucleus, Chemistry, General Neuroscience, food and beverages, DNA Methylation, nervous system diseases, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, Forebrain, Excitatory postsynaptic potential, Linker, Chromatin immunoprecipitation, Neuroscience, Protein Binding |
| الوصف: | Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent. |
| تدمد: | 1546-1726 1097-6256 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30d4ff1fe94ce93eb7343985dada4566Test https://doi.org/10.1038/s41593-018-0155-8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30d4ff1fe94ce93eb7343985dada4566 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15461726 10976256 |
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